Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments

Xu-ran MA; Yan-li WANG; Di-xin ZOU; Dun-fang WANG; Hong-xin SONG; Wei-peng YANG; Yong-xiang GE

doi:10.13422/j.cnki.syfjx.20202004

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Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments

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- Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 1-8(2020)
- 作者机构：
  
  1.中国中医科学院中药研究所，北京 100700
  2.北京市中西医结合医院，北京 100039
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202004
  CLC： R2-0;R22;R285.5;R289
- Received：02 March 2020，
  
  Published Online：27 July 2020，
  
  Published：20 October 2020
- 稿件说明：
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马旭冉,王彦礼,邹迪新等.3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析[J].中国实验方剂学杂志,2020,26(20):1-8.

MA Xu-ran,WANG Yan-li,ZOU Di-xin,et al.Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):1-8.
马旭冉,王彦礼,邹迪新等.3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析[J].中国实验方剂学杂志,2020,26(20):1-8. DOI： 10.13422/j.cnki.syfjx.20202004.

MA Xu-ran,WANG Yan-li,ZOU Di-xin,et al.Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):1-8. DOI： 10.13422/j.cnki.syfjx.20202004.

摘要

目的

对比研究黄芩汤、四神丸、痛泻要方3首止泻名方对大鼠溃疡性结肠炎(UC)炎症反应、水运调节及脑肠轴的影响。

方法

42只SPF级雄性SD大鼠，以2

6-三硝基苯磺酸(TNBS)复合造模法制备UC大鼠模型，按体质量随机分为正常组、模型组、阳性药柳氮磺胺吡啶组(SASP，0.5 g·kg

-1

)，黄芩汤组(Huangqintang，HQT，20 g·kg

-1

)，四神丸组(Sishen Wan，SSW，26 g·kg

-1

)和痛泻要方组(Tongxie Yaofang，TXYF，22 g·kg

-1

)。连续给药5 d后，大鼠眼眶取血并处死取结肠，苏木素-伊红（HE）染色检测大鼠结肠组织病变损伤程度，酶联免疫吸附测定(ELISA)结合免疫组化(IHC)测定大鼠血清及结肠组织内血管活性肠肽(VIP)，5-羟色胺(5-HT)，P物质(SP)分布，蛋白免疫印迹法(Western blot)检测大鼠结肠组织内水通道蛋白3(AQP3)，水通道蛋白4(AQP4)蛋白水平，实时荧光定量聚合酶链式反应(Real-time PCR)检测大鼠结肠组织内细胞外调节蛋白激酶1(Erk1)，p38丝裂原活化蛋白激酶(p38 MAPK) mRNA表达。

结果

与正常组比较，模型组5-HT，VIP含量均显著下降(

0.01)，SP含量降低，但未见显著性统计学差异；与模型组比较，SASP组和TXYF组5-HT含量明显升高(

0.05)，SASP组，HQT组，TXYF组VIP，SP含量均明显升高(

0.05)。与正常组比较，模型组AQP3含量显著升高(

0.01)，AQP4含量显著降低(

0.01)；与模型组比较，HQT组AQP3含量显著降低(

0.01)，SASP组，HQT组AQP4含量明显升高(

0.05)；与模型组比较，各给药组Erk1，p38 MAPK mRNA表达均显著降低(

0.01)，HQT组降低趋势最为显著。

结论

3首名方对大鼠溃疡性结肠炎均有一定治疗作用，可有效改善UC大鼠的体征及精神状况，但在改善泄泻状态下脑肠轴紊乱方面，TXYF治疗作用优于HQT和SSW，在抑制炎症反应及调节水运平衡方面，HQT治疗作用优于TXYF和SSW。

Abstract

Objective

This study was designed to compare inflammatory response

water carriage and gut brain axis in rats with ulcerative colitis (UC) after treatment of three regiments

Huangqintang (HQT)

Sishenwan (SSW)

and Tongxie Yaofang(TXYF).

Method

After approved by Institute of Chinese Materia Medica Ethics Committees in China Academy of Chinese Medical Sciences

UC in rats was induced by using a compound method (trinitrobenzenesulfonic acid plus ethanol). Rats were randomly divided into control

disease

positive control salazosulfapyridine (SASP

0.5 g·kg

-1

)

HQT (20 g·kg

-1

)

SSW(26 g·kg

-1

)

and TXYF group(22 g·kg

-1

). After 5 days of treatment

colonic tissues and the blood were taken for various assays. Damage of colonic tissues was detected by hematoxylin-eosin staining (HE). The distribution of Vasoactine intrestinal (VIP)

5-hydroxytrytamine (5-HT)

P-substance (SP) in the blood and serum were detected by enzymelinked immunosorbent assay (ELISA) and immunohistochemistry (IHC)

the levels of aquaporin3 (AQP3) and Aquaporin4 (AQP4) in the serum were detected by Western blot

the mRNA expression of Extracellular regulated protein kinases 1 (Erk1) and p38 mitogen-activated protein kinase (p38 MAPK) in the serum were detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR).

Result

The brain-gut peptide results showed that compared with the normal group

the content of 5-HT and VIP in model group were significantly decreased (

0.01)

the content of SP were decreased

but there was no significant statistical difference

compared with the disease group

the content of 5-HT in SASP and TXYF group were clearly increased (

0.05)

the increment of VIP and SP in SASP

HQT

TXYF group were significant (

0.05). Compared with the normal group

the content of AQP3 in model group were significantly increased(

0.01)

the content of AQP4 were clearly decreased(

0.01)

compared with the disease group

the content of AQP4 in SASP and HQT group were clearly increased (

0.05)

whereas the levels of AQP3 in HQT group were most significant reduced (

0.01). Compared with the disease group

the expression of Erk1 and p38 were clearly reduced (

0.01)

with the most significant reduce being the expression in HQT group.

Conclusion

Three regiments all have therapeutic effects on UC

manifested by improvements of the signs and mental status of UC rats. However

in terms of gut-brain axis disturbance improvement

the therapeutic effect of TXYF was superior than HQT and SSW

whereas in terms of inflammatory response suppression and water carriage accomodation

the therapeutic effect of HQT was superior than SSW and TXYF.

关键词

Keywords

references

SHAPIRO J M , ZOEGA H , SHAH S A , et al . Incidence of Crohn's disease and ulcerative colitis in Rhode Island:report from the Ocean State Crohn's and Colitis Area Registry [J]. Inflamm Bowel Dis , 2016 , 22 ( 6 ): 1456 - 1461 .

LI X Y , DAI M T , CHAI K F . Analysis on the application of treating the same disease with different therapies and treating different disease with the same therapy in Jingui Yaolue [J]. Chin J Traditi Chin Med Pharm , 2017 , 32 ( 10 ): 4351 - 4354 .

LI T , ZHUANG S X , WANG Y W , et al . Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography [J]. Acta Pharm Sin B , 2016 , 6 ( 2 ): 148 - 157 .

SU J , LIU Y L , GUO Y , et al . Simulataneous determination of 7 active constituents in Si-Shen-Wan by multi-wavelength HPLC [J]. J of Drug Anal , 2013 , 33 ( 9 ): 1531 - 1537 .

HU Y , ZHENG Y L , MEI Q X , et al . Simultaneous determination of seven active ingredients in Tong-Xie-Yao-Fang and pieces by UPLC [J]. Tradit Chin Med And Clin Pharmacol , 2017 , 28 ( 6 ): 786 - 791 .

MA X R , WANG Y L , ZOU D X , et al . Comparison of intestinal bacteria and inflammatory cytokine expression in rats with ulcerative colitis after treatment of three regiments [J]. Acta Pharm Sin , 2019 , 54 ( 7 ): 1241 - 1250 .

LI X L , ZHANG Y J , LAI B Y , et al . Kangfuxin (Periplaneta americana) lotion for adjunctive treatment of ulcerative colitis: a systematic review and meta‑analysis of randomized controlled trials [J]. World J Tradit Chin Med , 2018 , 4 ( 3 ): 77 - 84 .

DELGADO M , GANEA D . Vasoactive intestinal peptide:a neuropeptide with pleiotropic immune functions [J]. Amino Acids , 2013 , 45 ( 1 ): 25 - 39 .

YU F Y , HUANG S G , ZHANG H Y , et al . Comparison of 5-hydroxytryptophan signaling pathway characteristics in diarrhea-predominant irritable bowel syndrome and ulcerative colitis [J]. World J Gastroenterol , 2016 , 22 ( 12 ): 3451 - 3459 .

FANG K , LAW I K , PADUA D , et al . MicroR%NA-31-3P is involved in substance P (SP) associated inflammation in human colonic epithelial cells and experimental colitis [J]. Am J Pathol , 2018 , 188 ( 3 ): 586 - 599 .

KREIDA S , TORNROTH-HORSEFIELD S . Structural insights into aquaporin selectivity and regulation [J]. Curr Opin Struct Biol , 2015 , 33 : 126 - 134 .

LAFORENZA U . Water channel proteins in the gastrointestinal tract [J]. Mol Asp Med , 2012 , 33 ( 5/6 ): 642 - 650 .

ZHAO G , LI J , WANG J , et al . Aquaporin 3 and 8 are down-regulated in TNBS-induced rat colitis [J]. Biochem Biophys Res Commun , 2014 , 443 ( 1 ): 161 - 166 .

JONATHAN J , HANSEN M D , LISA H , et al . Gene expression patterns in experimental colitis in IL-10 deficient mice [J]. Inflamm Bowel Dis , 2009 , 15 ( 6 ): 890 - 899 .

甘江华 , 黄玙璠 , 彭代银 , 等 . 3种石斛对脾阴虚型大鼠便秘的治疗作用及其机制初步研究 [J]. 中国中药杂志 , 2019 , 44 ( 12 ): 2600 - 2606 .

NAKAYAMA S , MIGLIATI E , AMIRY-MOGHADDAM M , et al . Osmotherapy with hypertonic saline attenuates global cerebral edema following experimental cardiacarrest via perivascular pool of aquaporin-4 [J]. Crit Care Med , 2016 , 44 ( 8 ): e702 - e710 .

KUNZELMANN K , MALL M . Electrolyte transport in the mammalian colon:mechanisms and inplications for disease [J]. Physiol Rev , 2002 , 82 ( 1 ): 245 - 289 .

BALIGA M S , JOSEPH N , VENKATARANGANNA M V , et al . Curcumin,an active compoment of tumeric in the prevention and treatment of ulcerative colitis: preclinical and clinical observations [J]. Food Funct , 2012 , 3 ( 11 ): 1109 - 1117 .

SETIA S , NEHRU B , SANYAL S N . Upregulation of MAPK/Erk and PI3K/Akt pathways in ulcerative colitis associated colon cancer [J]. Biomed Pharmacother , 2014 , 68 ( 8 ): 1023 - 1029 .

徐冬梅 , 陈勇 , 方朝晖 , 等 . 基于MAPK途径探讨丹蛭降糖胶囊改善高脂血症大鼠肝损伤的机制 [J]. 中国中药杂志 , 2019 , 44 ( 14 ): 2953 - 2959 .

YU L , YAN J , SUN Z . D-limonene exhibits anti-inflammatory and antioxidant properties in an ulcerative colitis rat model via regulation of iNOS,COX-2,PGE2 and ERK signaling pathways [J]. Mol Med Rep , 2017 , 15 ( 4 ): 2239 - 2346 .

张会会 . 黄芩汤抗炎药效及其作用机制研究 [D]. 北京 : 中国中医科学院 , 2014 .

WANG D F , WANG Y L , WANG Y W , et al . Effect of Huangqin Tang the function of regulatory TLR4/MyD88 signal pathway in rats with ulcerative colitis [J]. Acta Pharmaceutica Sinica , 2016 , 51 ( 10 ): 1558 - 1563 .

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