XU Jiao,HUANG Xuan,ZHOU Tao,et al.Effect of Gastrodia elata Planting on Soil Microbial Community Structure[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):10-16.
XU Jiao,HUANG Xuan,ZHOU Tao,et al.Effect of Gastrodia elata Planting on Soil Microbial Community Structure[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):10-16. DOI: 10.13422/j.cnki.syfjx.20202016.
Effect of Gastrodia elata Planting on Soil Microbial Community Structure
To analyze the changes of soil microbial community structure before and after planting
Gastrodia elata
in different producing areas,and to investigate the response of soil microorganisms to the planting of
G. elata
.
Method
2
ITS and 16S rDNA high-throughput sequencing technologies were used to detect fungal and bacterial community compositions in the soil,including the soil without planting
G. elata
(CK1,CK2),the soil around
G. elata
(GE1,GE2)before harvesting
and the soil around the rhizomorph of
Armillaria
(AGE1,AGE2) in Dafang
Guizhou and Jinzhai
Anhui respectively.
Result
2
Principal component analysis (PCA) showed that the soil microorganisms changed significantly after
G. elata
planting as compared with the control soil. The sequencing results showed that the planting of
G. elata
increased the OTUs number of fungi and bacteria. As compared with the control soil,the diversity and abundance of fungal and bacterial communities showed an increase trend after the cultivation of
G. elata
in soil of Dafang
Guizhou, such changes of fungal communities were not significant
but the abundance of soil bacteria communities increased in Jinzhai
Anhui as compared with the control soil. The abundance of genera
Ilyonectria
and
Nitrospira
increased,while genera
Russula
decreased significantly both in the soil of Guizhou and Anhui. Furthermore,the abundance of
Fusarium
and
Mortierella
increased significantly in the soil of Dafang
Guizhou.
Conclusion
2
The soil microorganisms were out of balance after planting of
G. elata
and the abundance of pathogenic microorganisms such as
Ilyonectria
and
Fusarium
increased,which may be related to the plant diseases and insect pests of
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