Effects of High Temperature Stress on Growth Characteristics of <italic style="font-style: italic">Armillaria</italic>

Xuan HUANG; Jiao XU; Tao ZHOU; Xiao-hong OU; Qing-song YUAN; Jin-qiang ZHANG; Wei-ke JIANG; Yuan YUAN; Xiao WANG; Da-hui LIU; Guang-wen ZHANG

doi:10.13422/j.cnki.syfjx.20202017

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Effects of High Temperature Stress on Growth Characteristics of Armillaria

Topic | 更新时间：2020-09-16

- Effects of High Temperature Stress on Growth Characteristics of Armillaria
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 19, Pages: 17-22(2020)
- 作者机构：
  
  1.贵州中医药大学，贵阳 550025
  2.中国中医科学院中药资源中心，北京 100700
  3.山东省分析测试中心，济南 250014
  4.湖北中医药大学，武汉 430065
  5.贵州乌蒙腾菌业有限公司，贵州大方 551600
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202017
  CLC： R284.2;R289;R22;R2-031
- Received：16 February 2020，
  
  Published Online：23 July 2020，
  
  Published：05 October 2020
- 稿件说明：
移动端阅览
黄萱,徐娇,周涛等.高温胁迫对蜜环菌生长特性的影响[J].中国实验方剂学杂志,2020,26(19):17-22.

HUANG Xuan,XU Jiao,ZHOU Tao,et al.Effects of High Temperature Stress on Growth Characteristics of Armillaria[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):17-22.
黄萱,徐娇,周涛等.高温胁迫对蜜环菌生长特性的影响[J].中国实验方剂学杂志,2020,26(19):17-22. DOI： 10.13422/j.cnki.syfjx.20202017.

HUANG Xuan,XU Jiao,ZHOU Tao,et al.Effects of High Temperature Stress on Growth Characteristics of Armillaria[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):17-22. DOI： 10.13422/j.cnki.syfjx.20202017.

摘要

目的

探究高温胁迫对不同蜜环菌生长特性的影响，为筛选耐高温的优良蜜环菌菌株提供指导。

方法

以14株不同天麻产区的蜜环菌为实验材料，观察各蜜环菌菌株的生长特性并进行表型分类；采用rDNA-IGS序列分析进行分子鉴定，进一步确定供试菌株的亲缘关系；以23 ℃条件为对照（CK），30 ℃为高温胁迫，记录各菌株生长速度，生物量，菌索长度等指标。

结果

14株蜜环菌均与高卢蜜环菌

Armillaria gallica

相似性最高，亲缘关系最相近；但不同天麻产区的蜜环菌菌株生长特性存在明显差异，将14株蜜环菌分为Ⅳ个类群，其中生长状态：第Ⅰ类群

第Ⅱ类群

第Ⅲ类群

第Ⅳ类群；高温胁迫后，各菌株对高温的耐受性也存在明显差异，表现在菌索平均生长速度GZ16

SX1

GZ1；相对菌索长度GZ16

SX1

GZ3；相对生物量GZ16

SX4

GZ1

HB1

AH2；生长状态GZ16

SX1

GZ1。

结论

高温胁迫下，GZ16在生长速度，相对菌索长度，相对生物量及生长状态等均最优，SX1，GZ1菌株次之。表明菌株GZ16，SX1，GZ1耐高温能力较强，且这3个菌株在正常温度下生长特性也优良，适宜全国主要天麻产区生产。

Abstract

Objective

To explore the effects of high temperature stress on the growth characteristics of different

Armillaria

strains，and to provide guidance for screening excellent

Armillaria

strains with high-temperature resistance.

Method

14 strains of

Armillaria

from different

G. elata

producing areas were used as experimental materials to observe the growth characteristics and conduct phenotypic classification for the strains. rDNA-IGS sequence analysis was used for molecular identification to further determine the genetic relationship of the tested strains.The strain growth rate

biomass，mycelial length and other indicators under the condition of 23 ℃ （CK） and 30 ℃ high temperature stress were recorded.

Result

All the 14 strains of

Armillaria

had the highest similarity and the closest relationship with

Armillaria gallica

，but there were significant differences in growth characteristics among different

G. elata

producing areas. The 14 strains of

Armillaria

were classified into Ⅳ groups，and the growth status was groupⅠ

group Ⅱ

group Ⅲ

group Ⅳ. After treatment with high temperature stress，the tolerance of each strain to high temperature also showed obvious differences，as shown in the average growth rate of the mycelial was GZ16

SX1

GZ1. The rank of relative mycelial length was GZ16

SX1

GZ3 and the relative biomass was GZ16

SX4

GZ1

HB1

AH2.

Conclusion

Under high temperature stress，GZ16 was best in growth rate，relative length of mycelial，relative biomass and growth state，followed by SX1 and GZ1 strains. The results indicate that strains GZ16，SX1 and GZ1 have the strong resistance to high temperature and excellent growth characteristics at normal temperature，so these three strains are suitable to be produced in main

G. elata

producing areas in China.

关键词

Keywords

references

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Effects of High Temperature Stress on Growth Characteristics of Armillaria

DOI：10.13422/j.cnki.syfjx.20202017

摘要

Abstract

关键词

Keywords

references