Application of SmartRoot in Phenotype Profile Analysis of <italic style="font-style: italic">Armillaria gallica</italic>

Xue-yan ZHANG; Qi-tiao HU; Cheng-wu FANG; Yuan YUAN

doi:10.13422/j.cnki.syfjx.20202019

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Application of SmartRoot in Phenotype Profile Analysis of Armillaria gallica

Topic | 更新时间：2020-09-16

- Application of SmartRoot in Phenotype Profile Analysis of Armillaria gallica
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 19, Pages: 23-28(2020)
- 作者机构：
  
  1.安徽中医药大学药学院，合肥 230012
  2.中国中医科学院中药资源中心，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202019
  CLC： R284.2;R289;R22;R2-031
- Received：13 January 2020，
  
  Published Online：23 July 2020，
  
  Published：05 October 2020
- 稿件说明：
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张雪艳,胡启跳,方成武等.SmartRoot在蜜环菌菌索表型谱分析中的应用[J].中国实验方剂学杂志,2020,26(19):23-28.

ZHANG Xue-yan,HU Qi-tiao,FANG Cheng-wu,et al.Application of SmartRoot in Phenotype Profile Analysis of Armillaria gallica[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):23-28.
张雪艳,胡启跳,方成武等.SmartRoot在蜜环菌菌索表型谱分析中的应用[J].中国实验方剂学杂志,2020,26(19):23-28. DOI： 10.13422/j.cnki.syfjx.20202019.

ZHANG Xue-yan,HU Qi-tiao,FANG Cheng-wu,et al.Application of SmartRoot in Phenotype Profile Analysis of Armillaria gallica[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):23-28. DOI： 10.13422/j.cnki.syfjx.20202019.

摘要

目的

建立获取精确的表型谱数据研究平台，是目前中药资源研究亟待解决的技术难点。如传统的蜜环菌菌索表型表征方式多为描述性文字，存在较大的主观性和经验性，亟需一种客观并准确的方法进行蜜环菌菌索表型的表征。

方法

基于图像处理软件Image J和根系识别插件SmartRoot与Synbiosis ProtoCol 3影像分析仪相结合，对蜜环菌平板生长图片进行分析，测定蜜环菌菌索长度、生长速度、分枝情况、新生菌索夹角等，建立蜜环菌菌索表型谱分析测量体系。

结果

基于该文开发的方法，可以在不影响蜜环菌生长情况下，实时分析蜜环菌菌索的生长长度、生长速度、分枝数、新生菌索夹角等表型谱变化，继代后9~12 d的蜜环菌生长最为迅速，新生菌索与母体菌索之间夹角接近垂直。该方法与传统的蜜环菌生物量表型参数干重呈现一定的相关性，具有较好的实际应用价值。

结论

该研究建立了一套客观、准确、快捷和实时的蜜环菌菌索表型谱分析测量体系，拓展了SmartRoot的应用范围，可用于受控实验条件下中药资源的表型谱分析。

Abstract

Objective

To establish a research platform for obtaining accurate phenotypic spectrum data is a technical difficulty that needs to be resolved in the research of traditional Chinese medicine resources. For example，the traditional phenotypic characterization method of

Armillaria

rhizomorph is mostly in a form of descriptive text，which is subjective and empirical. There is an urgent need for an objective and accurate method to characterize the phenotype of honey fungus rhizomorph.

Method

Based on the image processing software Image J and the root identification plug-in SmartRoot combined with the Synbiosis ProtoCol 3 image analyzer，the growth picture of

Armillaria

spp. was analyzed，and the length，growth rate，branching situation，and angle of nascent rhizomorph of

Armillaria gallica

were measured to establish a measurement system for the phenotypic analysis of

Armillaria

rhizomorph.

Result

Based on the method developed in this paper，the growth length，growth rate，number of branches，angle of nascent rhizomorph，and other phenotypic changes can be analyzed in a real-time manner without affecting the growth of

Armillaria gallica

Armillaria

spp. grew fastest at 9-12 days after generation，and the angle between the nascent rhizomorph and the parent rhizomorph was nearly vertical. This method had a certain correlation with the dry weight of traditional

Armillaria

biomass phenotypic parameters，with a high value in practical application.

Conclusion

This study has established an objective，accurate，fast and real-time phenotypic analysis and measurement system for

Armillaria

rhizomorph，which expands the scope of application of SmartRoot and can be used for phenotypic analysis of traditional Chinese medicine resources under controlled experimental conditions.

关键词

Keywords

references

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Application of SmartRoot in Phenotype Profile Analysis of Armillaria gallica

DOI：10.13422/j.cnki.syfjx.20202019

摘要

Abstract

关键词

Keywords

references