Effect of Puerariae Lobatae Radix on Expression of Endoplasmic Reticulum Stress Related Proteins GRP78 and ATF6 in T2DM Rats

Lu-yu ZHANG; Yu-hui LIU; Yu YOU; Rong-hua LIU; Hao-kun LIU; Hai-bei WANG; Hai-hua HUANG

doi:10.13422/j.cnki.syfjx.20202036

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Effect of Puerariae Lobatae Radix on Expression of Endoplasmic Reticulum Stress Related Proteins GRP78 and ATF6 in T2DM Rats

Pharmacology | 更新时间：2020-09-16

- Effect of Puerariae Lobatae Radix on Expression of Endoplasmic Reticulum Stress Related Proteins GRP78 and ATF6 in T2DM Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 82-87(2020)
- 作者机构：
  
  1.江西中医药大学药学院，南昌 330004
  2.南昌大学第一附属医院，南昌 330006
  3.赣南医学院，江西赣州 341000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202036
  CLC： R2-0;R289;R587.1
- Received：28 April 2020，
  
  Published Online：13 August 2020，
  
  Published：20 October 2020
- 稿件说明：
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张路煜,刘玉晖,游宇等.葛根对2型糖尿病大鼠胰腺内质网应激相关蛋白GRP78，ATF6表达的影响[J].中国实验方剂学杂志,2020,26(20):82-87.

ZHANG Lu-yu,LIU Yu-hui,YOU Yu,et al.Effect of Puerariae Lobatae Radix on Expression of Endoplasmic Reticulum Stress Related Proteins GRP78 and ATF6 in T2DM Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):82-87.
张路煜,刘玉晖,游宇等.葛根对2型糖尿病大鼠胰腺内质网应激相关蛋白GRP78，ATF6表达的影响[J].中国实验方剂学杂志,2020,26(20):82-87. DOI： 10.13422/j.cnki.syfjx.20202036.

ZHANG Lu-yu,LIU Yu-hui,YOU Yu,et al.Effect of Puerariae Lobatae Radix on Expression of Endoplasmic Reticulum Stress Related Proteins GRP78 and ATF6 in T2DM Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):82-87. DOI： 10.13422/j.cnki.syfjx.20202036.

摘要

目的

观察葛根对2型糖尿病（T2DM）大鼠的疗效及其相关作用机制。

方法

90只SD大鼠分为正常组、模型组、二甲双胍组、葛根高、中、低剂量组，每组15只。对非正常组大鼠高脂高糖饲料喂养4周后采用一次性腹腔注射链脲佐菌素（STZ）40 mg·kg

-1

制造T2DM模型。葛根高、中、低剂量组分别灌胃葛根浸膏2.1，1.4，0.7 g·kg

-1

，二甲双胍组灌胃盐酸二甲双胍0.2 g·kg

-1

，正常组和模型组灌胃等体积蒸馏水，1次/d。灌胃8周后，测量大鼠空腹血糖（FBG），糖化血清蛋白（GSP），胰岛素（FINS），甘油三脂（TG），胆固醇（TC）含量，并计算胰岛素抵抗指数（HOMA-IR）；免疫组化观察胰腺组织中肿瘤坏死因子-

（TNF-

）的表达；蛋白免疫印迹法（Western blot）检测胰腺组织中葡萄糖调节蛋白78（GRP78），活化转录因子6（ATF6）蛋白的表达。

结果

与正常组比较，模型组的FBG，GSP，TG，TC，FINS，TNF-

含量明显增高（

0.05），HOMA-IR指数明显上升（

0.05），胰腺组织中GRP78，ATF6蛋白表达明显增高(

0.05)；与模型组比较，二甲双胍组、葛根高、中、低剂量组FBG，GSP，TG，TC，FINS，TNF-

含量均明显降低（

0.05），HOMA-IR指数明显降低（

0.05），胰腺组织中GRP78，ATF6蛋白表达明显降低（

0.05)。

结论

葛根可以显著改善T2DM大鼠胰岛素抵抗，抑制胰腺组织中炎症因子TNF-

的表达，减少胰腺组织中内质网应激相关蛋白GRP78，ATF6的表达。

Abstract

Objective

To observe the effect of Puerariae Lobatae Radix on type 2 diabetes mellitus (T2DM) rats and related mechanism.

Method

Ninety SD rats were divided into normal group

model group

metformin group

Puerariae Lobatae Radix high dose group

Puerariae Lobatae Radix medium dose group and Puerariae Lobatae Radix low dose group

15 rats in each group. The rats in abnormal group were fed with high-fat and high sugar diet for 4 weeks

and then T2DM model was established by intraperitoneal injection of 40 mg·kg

-1

streptozotocin (STZ). Puerariae Lobatae Radix high-dose group was intragastrically administered with 2.1 g·kg

-1

of Puerariae Lobatae Radix extract powder

Puerariae Lobatae Radix medium-dose group was intragastrically administered with 1.4 g·kg

-1

of Puerariae Lobatae Radix extract powder

Puerariae Lobatae Radix low-dose group was intragastrically administered with 0.7 g·kg

-1

of Puerariae Lobatae Radix extract powder

0.2 g·kg

-1

of metformin hydrochloride in metformin group

distilled water once a day in normal group and model group. After 8 weeks

fasting blood glucose (FBG)

glycosylated serum protein (GSP)

insulin (FINS)

triglyceride (TG)

cholesterol (TC) were measured

and insulin resistance index （HOMA-IR） was calculated. The expression of tumor necrosis factor -

(TNF-

) was observed by immunohistochemistry. Western blot was used to detect the protein expression of glucose regulatory protein 78 （GRP78）

activated transcription factor 6 （ATF6） in pancreatic tissue.

Result

Compared with normal group

the contents of FBG

GSP

FINS

TNF-

in model group were significantly increased (

0.05)

the HOMA-IR was significantly increased (

0.05)

the protein expressions of GRP78 and ATF6 in pancreatic tissue were significantly increased (

0.05). Compared with model group

the contents of FBG

GSP

FINS and TNF-

in the metformin group and Puerariae Lobatae Radix high

medium and low dose groups were significantly decreased (

0.05)

HOMA-IR decreased significantly (

0.05)

and the expression of GRP78 and ATF6 protein in pancreatic tissue decreased significantly (

0.05).

Conclusion

Puerariae Lobatae Radix can significantly improve insulin resistance in T2DM rats

inhibit the expression of inflammatory factor TNF-

in pancreatic tissue

reduce the protein expression of GRP78 and ATF6 in pancreatic tissue.

关键词

Keywords

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