Transcriptome-wide Identification and Expression Profiles of WRKY Transcription Factor in <italic style="font-style: italic">Prunella vulgaris</italic>

Yun-hao ZHU; Zhi-heng CHEN; Cheng-ming DONG

doi:10.13422/j.cnki.syfjx.20202067

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Transcriptome-wide Identification and Expression Profiles of WRKY Transcription Factor in Prunella vulgaris

Pharmacy Fundamentals | 更新时间：2020-09-16

- Transcriptome-wide Identification and Expression Profiles of WRKY Transcription Factor in Prunella vulgaris
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 146-152(2020)
- 作者机构：
  
  1.河南中医药大学药学院，郑州 450046
  2.呼吸疾病诊疗与新药研发河南省协同创新中心，郑州 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202067
  CLC： R22;R289;R2-031;R284.2
- Received：07 August 2019，
  
  Published Online：30 July 2020，
  
  Published：20 October 2020
- 稿件说明：
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朱畇昊,陈志恒,董诚明.基于转录组的夏枯草WRKY转录因子的鉴定和表达特征分析[J].中国实验方剂学杂志,2020,26(20):146-152.

ZHU Yun-hao,CHEN Zhi-heng,DONG Cheng-ming.Transcriptome-wide Identification and Expression Profiles of WRKY Transcription Factor in Prunella vulgaris[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):146-152.
朱畇昊,陈志恒,董诚明.基于转录组的夏枯草WRKY转录因子的鉴定和表达特征分析[J].中国实验方剂学杂志,2020,26(20):146-152. DOI： 10.13422/j.cnki.syfjx.20202067.

ZHU Yun-hao,CHEN Zhi-heng,DONG Cheng-ming.Transcriptome-wide Identification and Expression Profiles of WRKY Transcription Factor in Prunella vulgaris[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):146-152. DOI： 10.13422/j.cnki.syfjx.20202067.

摘要

目的

利用生物信息学方法从夏枯草（

Prunella vulgaris

）转录组数据中筛选，鉴定夏枯草WRKY转录因子，并对其蛋白特征、表达水平进行分析。

方法

从夏枯草转录组数据库中筛选WRKY转录因子，并对蛋白基序、理化性质、功能注释、系统进化、表达特性等进行分析，并预测其功能。

结果

从夏枯草中共获得23条WRKY转录因子序列。序列结构分析发现，夏枯草WRKY蛋白均包含一个保守的WRKYGQK模块，通过与拟南芥同源蛋白进行进化分析表明，夏枯草WRKY蛋白可分为I和Ⅱ型2种类型；I组共有7个成员；Ⅱ组共有16个成员，Ⅱ组可进一步分为Ⅱ-b（3个），Ⅱ-c（5个），Ⅱ-d（3个）和Ⅱ-e（5个）4个亚组。夏枯草WRKY蛋白的理化特性分析结果表明，WRKY蛋白的氨基酸数在85~599个之间；相对分子质量在9 527.5~66 438.4 Da；理论等电点在5.01~ 9.83；其中c13719.graph_c0，c32199.graph_c0，c24547.graph_c0，c37881.graph_c0等可能在夏枯草次生代谢产物合成调控方面发挥一定的作用；c32199.graph_c0，c26537.graph_c0，c23728.graph_c0等可能在夏枯草病原菌的识别和防御中起到作用。使用夏枯草转录组数据库分析了23条WRKY转录因子在其茎、果穗、叶片的表达特性，结果表明不同组织中WRKY基因的表达水平差异显著。

结论

该研究首次鉴定了夏枯草的WRKY转录因子家族结构特征和表达特性，为后研究讨夏枯草WRKY转录因子的功能提供了有用的信息。

Abstract

Objective

To identify WRKY genes from the transcriptome dataset of

Prunella vulgaris

by bioinformatics method

and analyze the protein characteristics and expression level of these genes.

Method

WRKY transcription factor were identified from the

P. vulgaris

transcriptome database，their motif，physical and chemical properties，functional annotations，family evolution and expression patterns were analyzed

and their functions were predicted.

Result

A total of 23 WRKY transcription factors were identified from

P. vulgaris

in this study by computational prediction method.Structural analysis found that WRKY proteins contained a highly conserved motif WRKYGQK. Phylogenetic analysis of WRKYs together with the homologous genes from

Arabidopsis thaliana

could be divided into two groups（group Ⅰ-Ⅱ）. There were 7 members in group Ⅰ，and 16 members in group Ⅱ

group Ⅱ was subdivided into five subgroups，namely group Ⅱb （3 members），Ⅱc（5 members），Ⅱd（3 members），Ⅱe（5 members）. The physical and chemical properties of WRKY protein showed that the amino acid number was between 85 and 599，the molecular weight was between 9 527.5-66 438.45 Da，the theoretical isoelectric point was between 5.01-9.83.Among them

c13719.graph_c0，c32199.graph_c0，c24547.graph_c0，c37881.graph_c0 may play a role in the regulation of secondary metabolitessynthesis of

P. vulgaris

.And c32199.graph_c0，c26537.graph_c0，c23728.graph_c0 may has an effect in identifying and defensing pathogens in

P. vulgaris

.The transcriptional profiles of these 23 WRKY genes in various tissues were investigated using transcriptome dataset.The results showed that the expression level of WRKY genes varied significantly in different tissues.

Conclusion

This study identifies the organization and transcriptional profiles of PmWRKY genes for the first time

so as to provide the helpful information for further studies of functions of WRKYs.

关键词

Keywords

references

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Transcriptome-wide Identification and Expression Profiles of WRKY Transcription Factor in Prunella vulgaris

DOI：10.13422/j.cnki.syfjx.20202067

摘要

Abstract

关键词

Keywords

references