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1.重庆三峡学院 生物与食品工程学院,重庆 404120
2.重庆市食品药品检验检测研究院,重庆 404020
3.三峡库区道地药材绿色种植与深加工重庆市工程研究中心,重庆 404120
Received:12 January 2025,
Accepted:21 March 2025,
Published Online:25 March 2025,
Published:20 July 2025
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袁林,任雪杨,王逾涵等.基于最小数据集评价溶磷真菌对太白贝母土壤肥力的影响[J].中国实验方剂学杂志,2025,31(14):171-179.
YUAN Lin,REN Xueyang,WANG Yuhan,et al.Evaluation of Effect of Phosphorus-solubilizing Fungi on Soil Fertility of Fritillaria taipaiensis Based on Minimum Data Set[J].Chinese Journal of Experimental Traditional Medical Formulae,2025,31(14):171-179.
袁林,任雪杨,王逾涵等.基于最小数据集评价溶磷真菌对太白贝母土壤肥力的影响[J].中国实验方剂学杂志,2025,31(14):171-179. DOI: 10.13422/j.cnki.syfjx.20250416.
YUAN Lin,REN Xueyang,WANG Yuhan,et al.Evaluation of Effect of Phosphorus-solubilizing Fungi on Soil Fertility of Fritillaria taipaiensis Based on Minimum Data Set[J].Chinese Journal of Experimental Traditional Medical Formulae,2025,31(14):171-179. DOI: 10.13422/j.cnki.syfjx.20250416.
目的
2
探究施加3种不同溶磷真菌肥料对太白贝母种植地土壤质量的影响,并对土壤质量进行量化评价,为太白贝母的菌肥开发利用提供依据。
方法
2
以种植太白贝母的栽培土壤为研究对象,分为8个组别,Z1组(塔宾曲霉,
Aspergillus tubingensis
)、Z2组(黑曲霉,
A. niger
)、Z3组(烟曲霉,
A. fumigatus
)、Z12组(塔宾曲霉和黑曲霉)、Z13组(塔宾曲霉和烟曲霉)、Z23组(黑曲霉和烟曲霉)、Z123组(塔宾曲霉、黑曲霉和烟曲霉)和CK组(不施加菌肥),测定不同溶磷真菌施加后栽培土壤的pH、理化性质、微生物数量、酶活性等17个指标,利用相关性分析(CA)、主成分分析(PCA)等方法建立太白贝母土壤质量评价最小数据集(MDS),评价土壤肥力。
结果
2
不同处理组对土壤基本理化指标的影响各异,Z3组土壤中细菌菌落数显著高于其他处理组,土壤速效钾和速效氮含量最高值出现在Z13组,速效磷、全磷和有机质含量最高值出现在Z123组。在筛选出的7个土壤评价MDS指标中,中性磷酸酶对土壤的质量影响最大。各施肥组的肥力评价均优于CK组,Z123组(0.746)
>
Z3组(0.617)
>
Z23组(0.535)
>
Z13组(0.533)
>
Z12组(0.408)
>
Z1组(0.397)
>
Z2组(0.338)
>
CK组(0.269),Z123组土壤肥力水平最高,CK组最低;全量数据集的土壤肥力指数(SFQI)与MDS的SFQI值均为极显著相关(
P
<
0.01),说明建立的MDS能够较好地代替全量数据指标。
结论
2
施加溶磷真菌肥料是提升太白贝母栽培土壤质量的有效方式之一,施加组合菌株处理组优于单一菌株处理组,且施加Z123组菌株对栽培土壤的改善效果最佳。
Objective
2
To investigate the effects of three different phosphorus-solubilizing fungal fertilizers on soil quality in the planting area of
Fritillaria taipaiensis
and quantitatively evaluate soil quality, providing a basis for the development and utilization of fungi fertilizers for
F. taipaiensis
.
Methods
2
The rhizosphere soil of
F. taipaiensis
was collected and allocated into eight groups: Z1 (
Aspergillus tubingensis
), Z2
(
Aspergillus niger
), Z3 (
Aspergillus fumigatus
), Z12
(
A. tubingensis
and
A. niger
), Z13 (
A. tubingensis
and
A. fumigatus
), Z23 (
A. niger
and
A. fumigatus
), Z123 (
A. tubingensis
,
A. niger
, and
A. fumigatus
), and CK (no bacterial fertilizer). Seventeen indicators including soil pH, physical and chemical properties, microbial counts, and enzyme activities were measured after different fungal fertilizers were applied. The correlation analysis, principal component analysis, and other methods were adopted to establish a minimum data set for evaluating soil quality.
Results
2
The effects of different treatment groups on the basic physical and chemical indicators of soil varied. The bacterial count in the Z3 group was significantly higher than that of other treatment groups. The highest available potassium and available nitrogen content in soil appeared in the Z13 group, and the highest available phosphorus, total phosphorus, and organic matter content appeared in the Z123 group. Among the 7 indicators selected by the minimum data set for evaluating soil quality, neutral phosphatase activity had the greatest impact on soil quality. The fe
rtility of each fertilization group was superior to that of the CK group, following the trend of Z123 group (0.746)
>
Z3 group (0.617)
>
Z23 group (0.535)
>
Z13 group (0.533)
>
Z12 group (0.408)
>
Z1 group (0.397)
>
Z2 group (0.338)
>
CK group (0.269). The soil fertility was the highest in Z123 group and the lowest in CK group. The SFQI values of the total data set and the minimum data set were correlated (
P
<
0.01), indicating that the established minimum data set could replace the total data set.
Conclusion
2
Applying fungal fertilizer is an effective way to improve the soil quality of
F. taipaiensis
. Applying combined fungal strains outperforms applying single strains, and the Z123 group has the best improvement effect on soil.
罗敏 , 邓才富 , 李品明 , 等 . 药用植物太白贝母研究进展 [J]. 中国野生植物资源 , 2021 , 40 ( 2 ): 42 - 45,56 .
LUO M , DENG C F , LI P M , et al . Research progress in medicinal plant Fritillaria taipaiensis P.Y.Li [J]. Chin Wild Plant Res , 2021 , 40 ( 2 ): 42 - 45,56 .
国家药典委员会 . 中华人民共和国药典:一部 [M]. 北京 : 中国医药科技出版社 , 2020 : 38 - 39 .
National Pharmacopoeia Committee . Pharmacopoeia of the People's Republic of China:A [M]. Beijing : China Medical Science and Technology Press , 2020 : 38 - 39 .
段宝忠 , 陈锡林 , 黄林芳 , 等 . 太白贝母资源学研究概况 [J]. 中国现代中药 , 2010 , 12 ( 4 ): 12 - 14 .
DUAN B Z , CHEN X L , HUANG L F , et al . Resource research of Fritillaria taipaiensis P.Y.Li [J]. Mod Chin Med , 2010 , 12 ( 4 ): 12 - 14 .
李志强 , 齐鹏 , 王雅芝 , 等 . 氮磷添加对黄土高原小麦土壤养分含量及生态化学计量特征的影响 [J]. 中国农学通报 , 2024 , 40 ( 10 ): 95 - 102 .
LI Z Q , QI P , WANG Y Z , et al . Effect of nitrogen and phosphorus addition on nutrient content and ecological stoichiometry characteristics of wheat soils in loess plateau [J]. Chin J Agric , 2024 , 40 ( 10 ): 95 - 102 .
刘英杰 , 张丽红 , 张宏 , 等 . 溶磷微生物在土壤磷循环中的作用研究进展 [J]. 微生物学通报 , 2023 , 50 ( 8 ): 3671 - 3687 .
LIU Y J , ZHANG L H , ZHANG H , et al . Role of phosphate solubilizing microorganisms in soil phosphorus cycle:A review [J]. Microbiol China , 2023 , 50 ( 8 ): 3671 - 3687 .
李洁 , 滑磊 , 任启文 , 等 . 冀西北3种植被恢复类型土壤理化性质差异及肥力评价 [J]. 生态环境学报 , 2020 , 29 ( 8 ): 1540 - 1546 .
LI J , HUA L , REN Q W , et al . Physicochemical properties difference and fertility evaluation of soil within three types vegetation restoration in northwest Hebei [J]. Ecol Envir Sci , 2020 , 29 ( 8 ): 1540 - 1546 .
刘选帅 , 孙延亮 , 安晓霞 , 等 . 施磷和接种解磷菌对紫花苜蓿光合特性及生物量的影响 [J]. 草业学报 , 2023 , 32 ( 3 ): 189 - 199 .
LIU X S , SUN Y L , AN X X , et al . Effects of phosphorus application and inoculation with arbuscular mycorrhizalfungi and phosphorus-solubilizing bacteria on the photosynthetic characteristics and biomass of alfalfa [J]. Acta Pra Sin , 2023 , 32 ( 3 ): 189 - 199 .
催玲宇 , 喻曼 , 乔宇颖 , 等 . 基于Web of Science数据库的土壤质量评价及其微生物指标研究趋势分析 [J]. 浙江农业学报 , 2023 , 35 ( 11 ): 2688 - 2697 .
CUI L Y , YU M , QIAO Y Y , et al . Research trend of soil quality assessment and microbiological indicators based on Web of Science database [J]. Acta Agric Zhejiangensis , 2023 , 35 ( 11 ): 2688 - 2697 .
丁明净 , 脱云飞 , 郑阳 , 等 . 基于最小数据集栗子坪自然保护区不同林分类型土壤质量评价 [J]. 土壤通报 , 2024 , 55 ( 5 ): 1215 - 1228 .
DING M J , TUO Y F , ZHENG Y , et al . Soil quality evaluation of different forest types in liziping nature reserve based on the minimum data set [J]. Chin J Soil Sci , 2024 , 55 ( 5 ): 1215 - 1228 .
刘湘君 , 乔冠宇 , 郭丰浩 , 等 . 基于最小数据集的黄淮海旱作区耕层土壤质量评价及障碍分析 [J]. 农业工程学报 , 2023 , 39 ( 12 ): 104 - 113 .
LIU X J , QIAO G Y , GUO F H , et al . Evaluation and obstacle analysis of topsoil quality in Huang-Huai-hai dry region based on minimum data set [J]. Trans Chin Soc Agric Eng , 2019 , 39 ( 12 ): 104 - 113 .
周文宇 , 杨小虎 , 杨海昌 , 等 . 基于最小数据集的典型绿洲农田土壤质量评价 [J]. 灌溉排水学报 , 2023 , 42 ( 7 ): 117 - 122 .
ZHOU W Y , YANG X H , YANG H C , et al . Assessing soil quality in oasis ecosystems using a minimum dataset [J]. J Irrig Drain , 2023 , 42 ( 7 ): 117 - 122 .
关松荫 . 土壤酶及其研究法 [M]. 北京 : 农业出版社 , 1986 : 260 - 376 .
GUAN S Y . Soil enzymes and their research methods [M]. Beijing : Agriculture Press , 1986 : 260 - 376 .
鲁如坤 . 土壤农业化学分析方法 [M]. 北京 : 中国农业科技出版社 , 2000 : 12 - 231 .
LU R K . Methods of soil agricultural chemical analysis [M]. Beijing : China Agriculture Technology Press , 2000 : 12 - 231 .
鲍士旦 . 土壤农化分析 [M]. 北京 : 中国农业出版社 , 2000 : 14 - 24 .
BAO S D . Soil agrochemical analysis [M]. Beijing : China Agriculture Press , 2000 : 14 - 24 .
罗玲 , 钟奇 , 刘伟 , 等 . 地面覆盖对避雨葡萄园土壤水分、温度及酶活性的影响 [J]. 核农学报 , 2020 , 34 ( 12 ): 2839 - 2849 .
LUO L , ZHONG QI , LIU W , et al . Effects of mulching on soil moisture,temperature and enzyme activities in rain-sheltered vineyard in soil quality evaluation [J]. J Nucl Agric Sci , 2020 , 34 ( 12 ): 2839 - 2849 .
李鑫 , 张文菊 , 邬磊 , 等 . 土壤质量评价指标体系的构建及评价方法 [J]. 中国农业科学 , 2021 , 54 ( 14 ): 3043 - 3056 .
LI X , ZHANG W J , WU L , et al . Construction and evaluation method of soil quality evaluation index system [J]. Sci Agric Sin , 2019 , 54 ( 14 ): 3043 - 3056 .
CHOUDHURY B , SATYABRATA M . Indexing soil properties through constructing minimum datasets for soil qualityassessment of surface and profile soils of intermontane valley(Barak,North East India) [J]. Ecol Indic , 2021 , 123 : 107369 .
刘引 , 颜鸿远 , 欧小宏 , 等 . 基于最小数据集的麻城菊花种植区土壤肥力质量评价 [J]. 中国中药杂志 , 2019 , 44 ( 24 ): 5382 - 5389 .
LIU Y , YAN H Y , OU X H , et al . Soil fertility quality evaluation of Chrysanthemum morifolium planting area in Macheng based on minimum data set [J]. China J Chin Mater Med , 2019 , 44 ( 24 ): 5382 - 5389 .
贾志兴 , 江磊 , 陈明 , 等 . 基于最小数据集权重(MDS)的稀土土壤质量评价研究 [J]. 环境生态学 , 2023 , 5 ( 1 ): 1 - 6 .
JIA Z X , JIANG L , CHEN M , et al . Evaluation of rare earth soil quality based on minimum data set weights (MDS) [J]. Environ Ecol , 2023 , 5 ( 1 ): 1 - 6 .
王相宜 , 黄红心 , 鞠鲤懋 , 等 . 不同肥料投入对有机菜田土壤质量的影响 [J]. 中国农业大学学报 , 2024 , 29 ( 6 ): 197 - 205 .
WANG X Y , HUANG H X , JU L M , et al . Effects of different fertilizer inputs on the soil quality in organic vegetable fields [J]. J China Agric Univ , 2024 , 29 ( 6 ): 197 - 205 .
宋洪炳 , 郭伟玲 . 基于最小数据集的江淮丘陵区土壤质量综合评价 [J]. 农业与技术 , 2023 , 43 ( 12 ): 93 - 98 .
SONG H B , GUO W L . Comprehensive evaluation of soil quality in Jianghuai hilly area based on minimum dataset [J]. Agric Technol , 2023 , 43 ( 12 ): 93 - 98 .
王一帆 , 陈小琴 , 李词周 , 等 . 亳州菊花生长与土壤肥力的关系 [J]. 土壤 , 2024 , 56 ( 2 ): 291 - 299 .
WANG Y F , CHEN X Q , LI C Z , et al . Soil fertility evaluation and its relationship with growth of Chrysanthemum morifolium in Bozhou,China [J]. Soils , 2024 , 56 ( 2 ): 291 - 299 .
王艺霖 , 梁尧 , 蔡红光 , 等 . 基于最小数据集的不同有机物料还田黑土土壤质量评价 [J]. 土壤通报 , 2024 , 55 ( 1 ): 68 - 75 .
WANG Y L , LIANG Y , CAI H G , et al . Soil quality evaluation of black soil under different returning treatments of organic materials based on minimum data set [J]. Chin J Soil Sci , 2024 , 55 ( 1 ): 68 - 75 .
袁野 , 王浩 , 罗洋 , 等 . 基于最小数据集评价耕作方式对黑土农田土壤质量的影响 [J]. 玉米科学 , 2023 , 31 ( 4 ): 148 - 157 .
YUAN Y , WANG H , LUO Y , et al . Evaluation of tillage practices on the soil quality of black soil farmland based on the minimum data set [J]. J Maize Sci , 2023 , 31 ( 4 ): 148 - 157 .
苟国花 , 樊军 , 王茜 , 等 . 基于最小数据集的青藏高原南北部不同土地利用方式土壤质量评价 [J]. 应用生态学报 , 2023 , 34 ( 5 ): 1360 - 1366 .
GOU G H , FAN J , WANG Q , et al . Soil quality evaluation of different land use patterns on the southern and northern Qinghai-Tibet Plateau based on minimal data set [J]. Chin J Appl Ecol , 2023 , 34 ( 5 ): 1360 - 1366 .
马琳 , 王梓欣 , 蒋靖怡 , 等 . 基于最小数据集的蕲春蕲艾种植区土壤肥力质量评价 [J]. 中国中药杂志 , 2022 , 47 ( 14 ): 3738 - 3748 .
MA L , WANG Z X , JIANG J Y , et al . Assessment of soil fertility in Artemisia argyi planting areas in Qichun county based on minimum data set [J]. China J Chin Mater Med , 2022 , 47 ( 14 ): 3738 - 3748 .
梅楠 , 谷岩 , 李德忠 , 等 . 基于最小数据集的吉林省黑土耕层土壤质量评价 [J]. 农业工程学报 , 2021 , 37 ( 12 ): 91 - 98 .
MEI N , GU Y , LI D Z , et al . Soil quality evaluation in topsoil layer of black soil in Jilin province based on minimum data set [J]. Trans Chin Soc Agric , 2021 , 37 ( 12 ): 91 - 98 .
冯瑞琦 , 潘萍 , 欧阳勋志 , 等 . 基于最小数据集的闽楠天然次生林土壤质量评价 [J]. 江西农业大学学报 , 2021 , 43 ( 3 ): 585 - 597 .
FENG R Q , PAN P , OUYANG X Z , et al . Soil quality evaluation of natural secondary forest of Phoebe bournei based on minimum data set [J]. Acta Agric Univ Jiangxiensis , 2021 , 43 ( 3 ): 585 - 597 .
王成 , 马杨明 , 王春雨 , 等 . 种植方式与施氮量对杂交籼稻养分吸收特性及根系活力的影响 [J]. 作物学报 , 2024 , 50 ( 12 ): 3069 - 3082 .
WANG C , MA Y M , WANG C Y , et al . Effects of cropping practices and nitrogen application on nutrient uptake characteristics and root vigor of hybrid indica rice [J]. Acta Agron Sin , 2024 , 50 ( 12 ): 3069 - 3082 .
王文武 , 朱万泽 , 李霞 , 等 . 基于最小数据集的大渡河干暖河谷典型植被土壤质量评价 [J]. 中国水土保持科学:中英文版 , 2021 , 19 ( 6 ): 54 - 59 .
WANG W W , ZHU W Z , LI X , et al . Soil quality assessment of typical vegetation in dry and warm valley of DaduRiver based on minimum data set [J]. Sci Soil Water Conserv , 2021 , 19 ( 6 ): 54 - 59 .
乔云发 , 钟鑫 , 苗淑杰 , 等 . 基于最小数据集的东北风沙土农田耕层土壤质量评价指标 [J]. 水土保持研究 , 2019 , 26 ( 4 ): 132 - 138 .
QIAO Y F , ZHONG X , MIAO S J , et al . Evaluation indicators of soil quality in plough layer of aeolian sandy land in northease china based on minimum data set [J]. Res Soil Water Conserv , 2019 , 26 ( 4 ): 132 - 138 .
刘莹 , 孙文松 , 李玲 , 等 . 不同农业改良措施对老参地土壤酶活性及微生物群落的影响 [J]. 江苏农业科学 , 2024 , 52 ( 6 ): 235 - 241 .
LIU Y , SUN W S , LI L , et al . Effects of different agricultural improvement measures on soil enzyme activities and microbial community in old ginseng field [J]. Jiangsu Agric Sci , 2024 , 52 ( 6 ): 235 - 241 .
李青 , 李其胜 , 谢昶琰 , 等 . 生物有机肥与生物质灰渣配施对连作甜瓜生长及土壤性质的影响 [J]. 土壤通报 , 2024 , 55 ( 5 ): 1386 - 1394 .
LI Q , LI Q S , XIE C Y , et al . Effects of combined application of bio-organic fertilizer and biomass ash on the growth of continuous cropping muskmelon and soil properties [J]. Chin J Soil Sci , 2024 , 55 ( 5 ): 1386 - 1394 .
刘松涛 , 田春丽 , 曹雯梅 . 不同土壤质地棉花根际微生物及酶活性与土壤理化性质相关性研究 [J]. 安徽农业科学 , 2020 , 48 ( 5 ): 165 - 167,181 .
LIU S T , TIAN C L , CAO W M . Study on the correlation between rhizosphere microorganism and enzyme activity of cotton with different soil texture and soil physicochemistry [J]. J Anhui Agric Sci , 2020 , 48 ( 5 ): 165 - 167,181 .
张迎珂 , 卢树昌 , 夏渝静 . 菌剂施用对夏填闲玉米吸收土壤磷素及其形态转化的影响 [J]. 江苏农业科学 , 2024 , 52 ( 17 ): 218 - 223 .
ZHANG Y K , LU S C , XIA Y J . Effects of microbial agent application on soil phosphorus uptake and morphological transformation in summer catch corn [J]. Jiangsu Agric Sci , 2024 , 52 ( 17 ): 218 - 223 .
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