Objective: to study practicability on separating passive absorption active constituents of aqueous extract of traditional Chinese medicine(TCM) by liposome liquid membrane extraction technology. Method: The aqueous extract of TCM such as Fructus Evodiae and Herba Ephedrae were extracted by liposome preparaed respectively by ammonium sulfate transmembrane gradients method

citric acid transmembrane gradients method and sodium glutamine transmembrane gradients method by liquid membrane extraction technology. The active constituents extracted by liposome were determined by RP-HPLC. Result: Active constituents such as ephedrine and evodiamine which were passively absorbed much could be extracted easily by liposome

and the extracted rates were all more than 50%. Active constituents such as glycyrrhizic acid

chlorogenic acid

puerarin and paeoniflorin which were passively absorbed little could be extracted hard by liposome

and the extracted rates were all no more than 10%. The extracted rates were affected by the factors such as varieties of liposome

ratio of liposome and aqueous extract of TCM

time of extracting and temperature of extracting. Conclusion: Separating active constituents of aqueous extract of TCM by liposome liquid membrane extraction technology is feasible.