Effect of Different Treating Principles and Formulas on Expression of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand mRNA in Steroid-induced Osteonecrosis of Femoral Head in Chichen

WAN Rong; LI Li; KONG Xiang-ying; XIAO Chun-sheng; JIANG Yi-ni; Lin Na; KONG Huan-yu; CHEN Wei-heng

doi:CNKI:11-3495/R.20110303.1347.004

您当前的位置：

首页 >

文章列表页 >

Effect of Different Treating Principles and Formulas on Expression of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand mRNA in Steroid-induced Osteonecrosis of Femoral Head in Chichen

更新时间：2024-01-04

- Effect of Different Treating Principles and Formulas on Expression of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand mRNA in Steroid-induced Osteonecrosis of Femoral Head in Chichen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 8, Pages: 149-153(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：CNKI:11-3495/R.20110303.1347.004
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
WAN Rong, LI Li, KONG Xiang-ying, et al. Effect of Different Treating Principles and Formulas on Expression of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand mRNA in Steroid-induced Osteonecrosis of Femoral Head in Chichen[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 149-153.
DOI：

WAN Rong, LI Li, KONG Xiang-ying, et al. Effect of Different Treating Principles and Formulas on Expression of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand mRNA in Steroid-induced Osteonecrosis of Femoral Head in Chichen[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 149-153. DOI： CNKI:11-3495/R.20110303.1347.004.

摘要

目的: 观察比较健脾化痰、活血通络(简称健脾)与补肾壮骨、活血通络(简称补肾)2种治法对激素性股骨头坏死鸡股骨头骨保护素(osteoprotegerin

OPG) 、NF-κB受体活化因子配基(receptor activator of nuclear factor-κB ligand

RANKL) mRNA表达的影响

探讨2者防治股骨头坏死的分子机制。方法: 64只来杭鸡

随机分为对照、模型、健脾和补肾组

除对照组外均胸肌注射甲基泼尼松琥珀酸钠(5.2 mg ·kg-1)

健脾及补肾组在造模同时ig给予相应中药(6

9 g ·kg-1 ·d-1)

连续16周。给药8周和16周后

分批处死动物

取双侧股骨头

原位杂交法检测股骨头内OPG

RANKL mRNA的表达情况

并计算其比值。结果: 与正常组相比

模型组8周和16周时股骨头内OPG阳性细胞数显著减少

分别为(778±189)

(696±108)个

RANKL分别为(717±164)

(519±162)个

明显增多(P<0.01);与模型组相比

健脾方在8周就可以明显上调OPG阳性细胞数达(1 049±211)个

同时也降低RANKL阳性细胞数至(454±166)个(P<0.05或P<0.01)

升高OPG/RANKL比值(P<0.05)

而补肾组此时作用不明显

16周时作用比健脾组强(P<0.05)。结论: 健脾化痰、活血通络和补肾壮骨、活血通络2种不同治法方药均可调节股骨头内OPG和RANKL的基因表达

这可能是其有效抑制破骨细胞分化、防治激素性股骨头坏死的作用机制之一;健脾化痰、活血通络方药起效时间相对较早。

Abstract

Objective: To investigate the effect of two treating principles and formulas

named 'invigorating spleen to remove phlem and promoting blood circulation to remove meridian obstruction’(Jianpi) and 'invigorate the kidney and promoting blood circulation to remove meridian obstruction’(Bushen)

on the expression of osteoprotegerin(OPG) and receptor activator of nuclear factor-κB ligand(RANKL) mRNA in steroid-induced osteonecrosis of the femoral head(SONFH)

as well as to explore and compare their mechanism of prevention and treatment for SONFH. Method: Sixty-four chickens were randomly divided into control group

model group

Jianpi group and Bushen group. Except the control group

the animal model of SONFH was established with injection of methylprednisolone in chest muscle of chickens. At 8th and 16th weeks

both sides of femoral heads were taken off to observe the expression of OPG and RANKL mRNA with in situ hybridization staining. Result: Compared with the control group

the expression of OPG mRNA was decreased and RANKL mRNA increased significantly in model group(P<0.01). Compared with model group

both in Jianpi and Bushen group

the expression of OPG mRNA was increased and RANKL mRNA decreased significantly

meanwhile the ratio of OPG to RANKL was increased(P<0.01 or P<0.001). In Bushen group

the change did not obvious at the 8th weeks

however

the changes were significant compared with that in Jianpi group at the 16th weeks. Conclusion: Both Jianpi and Bushen formula can regulate the expression of OPG and RANKL mRNA to inhibit bone destruction

thereby exert preventive and therapeutic effect for SONFH. Jianpi take action earlier than Bushen formula.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Traditional Chinese Medicine in Prevention and Treatment of Osteonecrosis of Femoral Head Based on OPG/RANK/RANKL Signaling Pathway： A Review

Research Advances of Traditional Chinese Medicine in Treating Osteonecrosis of Femoral Head by Regulating Bone MetabolismRelated Signaling Pathways

Clinical Analysis of "Different Diseases Saring Same Syndrome" Concept in End Stage of Different Hip Diseases

Effect of Bugu Fangshu Decoction on Osteoprotegerin Expession of Bone Tissues in Osteoporosis Rats

Related Author

LIU Xiaoting

LIU Jianjun

AN Wenbo

GONG Yusuo

YUAN Baohua

HUANG Kang

LIU Tongke

KANG Fuping

Related Institution

Gansu University of Chinese Medicine

Affiliated Hospital of Gansu University of Chinese Medicine

Gansu Provincial Hospital of Traditional Chinese Medicine

Graduate School，Anhui University of Chinese Medicine

Affiliated Hospital of Institute of Neurology， Anhui University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰