Changes in Gene Expression Profiles of Gastric Carcinoma after Treatment of Compound Qinglongyi Capsule

XU Qing-rui; ZHANG Shu-ming; ZHANG Jun-wei; XU Chen; WANG Wei-ming

doi:CNKI:11-3495/R.20110303.1349.008

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Changes in Gene Expression Profiles of Gastric Carcinoma after Treatment of Compound Qinglongyi Capsule

更新时间：2024-01-04

- Changes in Gene Expression Profiles of Gastric Carcinoma after Treatment of Compound Qinglongyi Capsule
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 8, Pages: 180-183(2011)
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- DOI：CNKI:11-3495/R.20110303.1349.008
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- Published：2011
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XU Qing-rui, ZHANG Shu-ming, ZHANG Jun-wei, et al. Changes in Gene Expression Profiles of Gastric Carcinoma after Treatment of Compound Qinglongyi Capsule[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 180-183.
DOI：

XU Qing-rui, ZHANG Shu-ming, ZHANG Jun-wei, et al. Changes in Gene Expression Profiles of Gastric Carcinoma after Treatment of Compound Qinglongyi Capsule[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 180-183. DOI： CNKI:11-3495/R.20110303.1349.008.

摘要

目的: 研究复方青龙衣胶囊体外对人胃癌细胞SGC-7901基因表达谱的影响

初步探讨其对胃癌细胞抑制作用的机制。方法: 设定5个不同药物浓度梯度(0.8×10-3

1×10-3

1.2×10-3

1.4×10-3

1.6×10-3 g ·L-1)预处理24 h

采用MTT法测定对人胃癌细胞SGC-7901的抑制作用;以药物半数抑制浓度预处理24 h

全基因芯片技术研究对胃癌基因表达谱的影响

并采用基因集合聚类分析法分析结果。结果: 以不同浓度的复方青龙衣处理胃癌细胞SGC-7901 24 h

结果显示该药对肿瘤细胞的生长呈现剂量依赖性的抑制作用

半数抑制浓度为1.27×10-3g ·L-1;芯片结果显示:胃癌肿瘤细胞SGC-7901经药物处理后总共筛选出78个差异表达基因

表达上调的基因有23个

表达下调的基因有55个。结论: 复方青龙衣胶囊对人胃癌细胞SGC-7901具有显著的抑制作用

并可能通过影响细胞周期基因的表达

使肿瘤细胞停滞于G1/S期发挥抗肿瘤作用。

Abstract

Objective: To study the changes in gene expression profiles of gastric carcinoma after treatment of Compound Qinglongyi Capsule and the potential mechanism of it. Method: MTT assay was used to observe the effect of Compound Qinglongyi Capsule at doses of 0.8×10-3

1×10-3

1.2×10-3

1.4×10-3

1.6×10-3g.L-1 accordingly on proliferation of SGC-7901 cells. The gene expression spectra were tested by gene chip technique

and the databases were analyzed by gene set analysis. Result: MTT results showed that after treated with different doses for 24 h

Compound Qinglongyi Capsule could obviously inhibit the proliferation of SGC-7901 cells in a dose-dependent manner. Compared with the untreated group

expressions of 78 genes were changed

among them 23 gene expression up-regulated

and 55 gene expression down-regulated. Conclusion: Cell proliferation in the carcinoma cell line SGC-7901 can be inhibited effectively by Compound Qinglongyi Capsule.

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