Objective: To find out the best prescription composition of reinforcing Qi and enriching blood by factorial design in hemorrhagic blood deficient mouse. Method: The factor A with 12 levels was composed with 12 of reinforcing Qi medicine

such as Ginseng Radix et Rhizoma

Panacis Quinquefolii Radix

Codonopsis Radix

Pseudostellariae Radix

Astragali Radix

Atractylodis Macrocephalae Rhizoma

Dioscoreas Rhizoma

Glycyrrhizae et Rhizoma Radix

Jujubae Fructus

Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis

Ganoderma and factor B with 6 levels was composed with 6 of enriching blood medicine

such as Angelicae Sinensis Radix

Rehmanniae Radix

Pblygoni Multiflori Radix

Paeoniae Alba Radix

Corii Asini Colla

Longan. The medicines composed 72 prescriptions of reinforcing Qi and enriching blood according to 12 × 6 factorial design. The hemorrhagic blood deficient model of mouse was established with 0.5mL orbital bleeding. Peripheral blood cells were counted after 1

4

7

10 d of modeling. The repeated measures of factorial design were used to analyze the data. Result: There was no interaction between reinforcing Qi medicine and enriching blood medicine.The difference in effect of the prescriptions of reinforcing Qi and enriching blood on RBC was significant at different time points(P<0.01). The difference in effect of different levels of reinforcing Qi on RBC

HGB and HCT was significant(P<0.01)

and the Astragali Radix was the best. The effect of enriching blood medicines on RBC

HGB and HCT was all significant

and Angelicae Sinensis Radix was the best. The composition of Astragali Radix and Angelicae Sinensis Radix showed significant effect of enriching blood. Conclusion: The influence of reinforcing Qi medicine on the prescriptions is more strong than that of the medicines of enriching blood. Composition of Astragali Radix with Angelicae Sinensis Radix appears to be the best compatibility for the efficacy.