Objective: To study the condition of agent in vitro transdermal of indirubin under different accepted liquid and penetration enhancers. Method: The influence of indirubin accumulated permeability and through percentage of indirubin on different acceptable solution and penetration enhancers was detected by HPLC and Franz’s diffusion cell with excised mouse skin as percutaneous permeation barrier. Result: The effect of permeability of indirubin with different accepted liquid was different

the best accepted liquid was 40% ethanol saline and the through percentage was 28.77%; the effect of permeability of indirubin with different penetration enhancers was different

the best penetration enhancer was 5% borneol and the through percentage was 50.23%. Conclusion: The best effect of permeability of indirubin was with the condition of 40% ethanol saline as the accepted liquid and 5% borneol as the penetration enhancers.