Objective: To establish a method of fingerprint analysis on Notoginseng Radix et Rhizoma Formula granules by HPLC and provide a reference for quality evaluation of Notoginseng Radix et Rhizoma Formula granules. Method: The chromatographic fingerprints were obtained by the Waters Xbridge C18 column (4.6 mm×250 mm

5 μm) with the gradient elution and the solvent system was composed of acetonitrile-water. The detective wavelength was set at 203 nm; the flow rate was 1.0 mL ·min-1 and the column temperature was maintained at 30 ℃. Result: Eighteen copossessing peaks were selected as the fingerprint peaks of Notoginseng Radix et Rhizoma Formula granules and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of Notoginseng Radix et Rhizoma Formula granules and the standard fingerprint. Conclusion: The method with good reproducibility is simple and accurate; it can be used as a quality control method for Notoginseng Radix et Rhizoma Formula granules.