Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay

DONG Xiu-ying; LV Qing-tao; ZHANG Guo-ying; LING Jian-ya; ZHANG Chang-kai

doi:CNKI:11-3495/R.20110321.1133.006

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Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay

更新时间：2024-01-04

- Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 10, Pages: 70-73(2011)
- 作者机构：
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- DOI：CNKI:11-3495/R.20110321.1133.006
  CLC：
- Published：2011
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DONG Xiu-ying, LV Qing-tao, ZHANG Guo-ying, et al. Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 70-73.
DOI：

DONG Xiu-ying, LV Qing-tao, ZHANG Guo-ying, et al. Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 70-73. DOI： CNKI:11-3495/R.20110321.1133.006.

摘要

目的: 研究九州虫草体外不同极性部位的抗氧化作用。方法: 采用DPPH法测定九州虫草石油醚(EVP)、乙酸乙酯(EVE)、正丁醇(EVB)和水(EVW)不同提取部位的抗氧化活性。结果: EVP

EVE

EVB

EVW均有DPPH自由基的清除能力

测得EVP

EVE

EVB和EVW清除DPPH的EC50值分别为190.2

141.4

151.6

195.4 mg ·L-1。结论: 九州虫草具有很强的抗氧化性

为进一步研究其抗氧化活性成分提供了科学依据。

Abstract

Objective: To investigate the antioxidant effect in vitro of Cordyceps kyushuensis. Method :Antioxidant capacity of the petroleum ether fraction(EVP);acetic ether fraction(EVE);n-butanol fraction(EVB)and water fraction(EVW)were determined using the DPPH assay in vitro. Result: EVP

EVE

EVB and EVW showed antioxidant capacity of eliminating DPPH free radicals

and the scavenging efficiency increase with the concentration increase. EC50 values of the EVP

EVE

EVB and EVW are 190.2

141.4

151.6

195.4 mg ·L-1 respectively. Conclusion: C. kyushuensis shows great oxidation resistance

and the results of the antioxidant effect of the EVP

EVE

EVB

EVW provide a scientific basis to the research of active constituent and the exploitation of C.kyushuensis.

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