Effects of Water Extract of Corni Fructus on Melanin Synthesis in Co-culture System of Human Melanocytes and Kertinocytes

WANG Xing-yan; WANG Tian-ye; CHEN Qiao-yun; ZHANG Ning; LIU Hui; WANG Ying-ying; CHEN Jing-hua

doi:CNKI:11-3495/R.20110420.0931.008

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Effects of Water Extract of Corni Fructus on Melanin Synthesis in Co-culture System of Human Melanocytes and Kertinocytes

更新时间：2024-01-04

- Effects of Water Extract of Corni Fructus on Melanin Synthesis in Co-culture System of Human Melanocytes and Kertinocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 12, Pages: 141-143(2011)
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- DOI：CNKI:11-3495/R.20110420.0931.008
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- Published：2011
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WANG Xing-yan, WANG Tian-ye, CHEN Qiao-yun, et al. Effects of Water Extract of Corni Fructus on Melanin Synthesis in Co-culture System of Human Melanocytes and Kertinocytes[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 141-143.
DOI：

WANG Xing-yan, WANG Tian-ye, CHEN Qiao-yun, et al. Effects of Water Extract of Corni Fructus on Melanin Synthesis in Co-culture System of Human Melanocytes and Kertinocytes[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 141-143. DOI： CNKI:11-3495/R.20110420.0931.008.

摘要

目的: 考察山茱萸水提取物对体外构建的人黑素瘤细胞(A375)与角质形成细胞(Hacat)单层混合共培养模型黑素合成的影响。方法: 将山茱萸水提取物制备成含生药0.75~12 g ·L-15个质量浓度

细胞给药

通过MTT法、NaOH裂解法、多巴氧化法分别测定给药前后A375细胞与Hacat细胞共培养模型的细胞活力、黑素含量和酪氨酸酶活性。结果: 与空白对照组相比

1.5

0.75 g ·L-1山茱萸水提取物对共培养体系的细胞增殖率影响不大

对共培养体系黑素生成均有显著差异(P<0.01)

对共培养体系酪氨酸酶活性有差异(P<0.05或P<0.01);与阳性对照药物熊果苷相比

3 g ·L-1山茱萸水提取物对共培养体系黑素生成有显著差异(P<0.01)

作用强于熊果苷

1.5

0.75 g ·L-1山茱萸水提取物对共培养体系黑素生成无差异;与阳性对照药物熊果苷相比

1.5

0.75 g ·L-1山茱萸水提取物对共培养体系酪氨酸酶活性无差异。结论: 山茱萸水提取物能够通过抑制酪氨酸酶活性来抑制A375细胞和Hacat细胞共培养体系的黑素合成

为临床使用山茱萸治疗黄褐斑提供了实验依据。

Abstract

Objective: To evaluate the effects of water extract of Corni Fructus on the melanin synthesis in the co-culture system of human melanocytes and kertinocytes. Method: The proliferation

the tyrosinase activity and melanin contents of A375 and Hacat co-culture cells were measured by MTT

dopaoxidase and NaOH methods

respectively. Result: The tyrosinase activity and melanin content were decreased significantly (P<0.05) by the water extract of Corni Fructus. Dose dependence of the water extract was observed from 0.75 -12 g ·L-1. But the cell proliferation was not inhibited markedly (P<0.01). Conclusion: The melanin synthesis of A375 and Hacat co-culture cell system was decreased significantly by water extract of Corni Fructus. And tyrosinase activity inhibition was proved to be the mechanism of melanin decrease.

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