Effects of Shugan Liangxue Decoction on MCF-7 and T47D Cells Proliferation, Enzymatic Activity and mRNA Expression of Steroid Sulfatase

FU Xue-song; LI Ping-ping

doi:CNKI:11-3495/R.20110524.1326.001

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Effects of Shugan Liangxue Decoction on MCF-7 and T47D Cells Proliferation, Enzymatic Activity and mRNA Expression of Steroid Sulfatase

更新时间：2024-01-04

- Effects of Shugan Liangxue Decoction on MCF-7 and T47D Cells Proliferation, Enzymatic Activity and mRNA Expression of Steroid Sulfatase
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 14, Pages: 144-147(2011)
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- DOI：CNKI:11-3495/R.20110524.1326.001
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- Published：2011
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FU Xue-song, LI Ping-ping. Effects of Shugan Liangxue Decoction on MCF-7 and T47D Cells Proliferation, Enzymatic Activity and mRNA Expression of Steroid Sulfatase[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 144-147.
DOI：

FU Xue-song, LI Ping-ping. Effects of Shugan Liangxue Decoction on MCF-7 and T47D Cells Proliferation, Enzymatic Activity and mRNA Expression of Steroid Sulfatase[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 144-147. DOI： CNKI:11-3495/R.20110524.1326.001.

摘要

目的: 观察舒肝凉血方对雌激素受体阳性乳腺癌MCF-7和T47D细胞增殖的影响

并检测舒肝凉血方对两个细胞系中硫酸酯酶的活性及其mRNA表达的影响。方法 :培养雌激素受体阳性的乳腺癌细胞MCF-7和T47D

给予不同浓度的舒肝凉血方处理24 h后

MTT法检测复方对MCF-7和T47D细胞增殖的影响;RT-PCR检测舒肝凉血方对硫酸酯酶mRNA表达的影响。培养MCF-7和T47D细胞

转染萤火虫荧光素酶和海肾荧光素酶质粒

给予不同浓度的舒肝凉血方处理24 h后

双荧光素酶报告基因检测试剂盒检测复方对两个细胞系中硫酸酯酶活性的影响。结果 :舒肝凉血方显著抑制了MCF-7和T47D细胞的增殖

并呈现出剂量依赖关系

半数抑制浓度(IC50)分别是46.08 g ·L-1 和22.98 g ·L-1;低浓度的舒肝凉血方对硫酸酯酶mRNA的表达无明显影响

高浓度时则显著下调了硫酸酯酶mRNA的表达;舒肝凉血方显著降低了MCF-7和T47D细胞中硫酸酯酶的活性

且呈现出剂量依赖性。结论 :舒肝凉血方能显著抑制MCF-7和T47D细胞的增殖

此作用可能与该方降低硫酸酯酶活性以及下调硫酸酯酶mRNA的表达有关。

Abstract

Objective: To investigate the effects of Shugan Liangxue decoction (SGLXD) on MCF-7 and T47D cells proliferation

and evaluate the effects of SGLXD on the enzymatic activity and mRNA expression of steroid sulfatase (STS). Method: Estrogen receptor positive breast cancer MCF-7 and T47D cells were cultured and treated with different concentrations of SGLXD for 24 h. Cell proliferation was evaluated by MTT assay

and the mRNA expression of STS was investigated by RT-PCR. After transient co-transfection with plasmid pERE-TK-Luc and pRL-TK in MCF-7 and T47D cells

and treated with different concentrations of SGLXD for 24 h

we evaluated the enzymatic activity of STS by dual-luciferase reporter assay (DLR). Result : By MTT assay

SGLXD inhibited the cell proliferation of MCF-7 and T47D dose-dependently

the IC50 values were 46.08 g ·L-1 and 22.98 g ·L-1. SGLXD down-regulated the expression of STS mRNA at higher concentrations significantly. In MCF-7 and T47D cells

the enzymatic activity of STS was significantly inhibited by SGLXD dose-dependently. Conclusion : SGLXD can inhibit the cell proliferation of MCF-7 and T47D

this effect may be associated with the inhibitory effects of SGLXD on the enzymatic activity of STS and the expression of STS mRNA.

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