WU Li-ping, DENG Shi-gui, HUANG Hai-ding. Effect of Rhei Radix et Rhigoma on AQP mRNA Expression of Alveolar Type Ⅱ Cell Injured by LPS[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 213-217.
DOI:
WU Li-ping, DENG Shi-gui, HUANG Hai-ding. Effect of Rhei Radix et Rhigoma on AQP mRNA Expression of Alveolar Type Ⅱ Cell Injured by LPS[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 213-217. DOI: CNKI:11-3495/R.20110622.1303.003.
Effect of Rhei Radix et Rhigoma on AQP mRNA Expression of Alveolar Type Ⅱ Cell Injured by LPS
Objective: To observe effect of Rhei Radix et Rhigoma on AQP1
5 mRNA expression of alveolar type Ⅱcell(ATⅡ) injured by LPS. Method: Primary cultured alveolar type Ⅱcells were divided into 5 groups: control group: 2mL DMEM cultured medium was added; LPS injured cell model group(acute lung injury cell model group): 10 μg·mL-1 LPS was added to injure AT Ⅱ cell; serum containing Rhei Radix et Rhigoma intervention group was divided into 3 groups: each group was added the total volume of 5%
10%
20% serum containing Rhei Radix et Rhigoma. Which were collected After 4 hours of culture the mRNA expression of AQP1
AQP5 was detected by Real-Time PCR. Result: Compared with the control group
AQP1 mRNA expression of LPS injured cell model group was significantly lower (P<0.05). Compared with the LPS group
20% of total volume of serum containing Rhei Radix et Rhigoma intervention group could increase AQP1 mRNA expression significantly
but not return to the normal level. Compared with the control group
AQP5 mRNA expression of LPS injured cell model group was increased. Rhei Radix et Rhigoma intervention groups could decrease AQP5 mRNA expression
but not significantly. Conclusion: The protective mechanism of Rhei Radix et Rhigoma on acute lung injury cell model by LPS may be associated with the increased AQP1 mRNA expression and the decreased AQP5 mRNA expression.
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