Studies on HPLC Fingerprint of Heishunpian from Different Origin

HUANG Hui-lian; LIU Ke-lan; SHAO Feng; REN Gang; DUAN Feng; LIU Rong-hua

doi:CNKI:11-3495/R.20110622.1304.005

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Studies on HPLC Fingerprint of Heishunpian from Different Origin

更新时间：2024-01-04

- Studies on HPLC Fingerprint of Heishunpian from Different Origin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 16, Pages: 44-49(2011)
- 作者机构：
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- DOI：CNKI:11-3495/R.20110622.1304.005
  CLC：
- Published：2011
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HUANG Hui-lian, LIU Ke-lan, SHAO Feng, et al. Studies on HPLC Fingerprint of Heishunpian from Different Origin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 44-49.
DOI：

HUANG Hui-lian, LIU Ke-lan, SHAO Feng, et al. Studies on HPLC Fingerprint of Heishunpian from Different Origin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 44-49. DOI： CNKI:11-3495/R.20110622.1304.005.

摘要

目的: 研究四川江油、陕西汉中及云南丽江等附子主产区黑顺片的高效液相色谱指纹图谱

为建立附子的行业质量标准提供依据。方法: 利用HPLC方法

色谱条件Alltimate C18色谱柱(4.6 mm×250 mm

5 μm)

柱温35 ℃

流动相40 mmol·L-1 醋酸铵(氨水调pH 10.00)-乙腈梯度洗脱

流速1.0 mL·min-1

检测波长为240 nm。对不同主产地的22批黑顺片进行研究

采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A和聚类分析对指纹图谱进行了分析。结果: 22批黑顺片有11个共有峰

江油地区(道地产区)的黑顺片之间的相似度均大于0.900

非道地产区与道地产区的相似度小于0.900。结论: 该方法精密度、重复性和稳定性良好

可结合黑顺片含量测定用于全面控制附子炮制品(黑顺片)的质量。

Abstract

Objective: To study the fingerprints of Heishunpian from Sichuan Jiangyou

Shanxi Hanzhong and Yunnan Lijiang by high performance liquid chromatographic and establish the method of standard quality for Radix Aconiti Lateralis Praeparata. Method: HPLC method was used to establish the fingerprints of 22 batches of Heishunpian. Alltimate C18 column (4.6 mm×250 mm

5 μm) was used

and ammonium acetate solution-acetonitrile as the mobile phase

with a flow rate of 1.0 mL·min-1 and a detection wavelength at 240 nm. The obtained data was analyzed by computer aided similarity evaluation and cluster analysis software. Result: There were 11 common peaks in 22 habitats of Heishunpian. The similarity degree of the products produced in Jiangyou was found to be higher than 0.900. Conclusion: The method is accurate

repeatable and reliable

which can be used for their quality control.

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Related Author

WU Yong1

HE Fu-yuan1

CAO Yan2

XU Feng-qing1

WANG Ning1

LIU Jin-qi1

ZHOU An1

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

Tianjin University of Traditional Chinese Medicine

Modern Research Center for Traditional Chinese Medicine，Shanxi University

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