LI Fang, SUN Lan, TANG Chuan-lin, et al. Effect of Reinforcing Kidney and Activating Blood Prescription on Autoimmune Premature Ovarian Failure in Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 137-140.
DOI:
LI Fang, SUN Lan, TANG Chuan-lin, et al. Effect of Reinforcing Kidney and Activating Blood Prescription on Autoimmune Premature Ovarian Failure in Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 137-140. DOI: CNKI:11-3495/R.20110622.1305.006.
Effect of Reinforcing Kidney and Activating Blood Prescription on Autoimmune Premature Ovarian Failure in Mice
Objective: To investigate the therapeutical effect of a Chinese prescription with the function of reinforcing kidney and activating blood on immune premature ovarian failure (POF) in mice. Method: Each female BALB/c mouse was received multipoint immunization of porcine ZP4 (pZP4) in two hind footpads and intra-cutaneously to made the POF model. The mice were randomly divided into three Chinese medicine groups
contorl group
model group and estradiol (E2) group. After administrating Chinese medicine in three different doses (15.0
7.5
3.75 g·kg-1)
0.5% CMC-Na and E2 were given respectively for 20 days
vaginal smearses were visualized microscopically. E2 and pZP4 antibody levels were monitored. The morphology of ovarian tissue inflammation were studied. Result: Compared to the control group
the sex cycles of the high dose group was between 6 and 7 days without apparent change. Compared to the model group
the high dose group developed significantly higher serum E2 level (P<0.01) and lower serum antiboides to pZP4 (P<0.05)
and moreover
the number of primary follicles and secondary follicles were increased significantly (P<0.05). Conclusion: The Chinese prescription can improve the ovarian reproductive endocrine function and regulate the developmental capacity of ovarian follicle and corpus luteum in the four cycles. Otherwise
the Chinese medicine might inhibit the autoimmune presenility POF to some extent.
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