Objective: To study Yiqi Huoxue Jiangzhuo decoction (YHJD) on transforming growth factor-beta 1(TGF-β1)

smad7

p-smad2/3

integrin-linked kinase(ILK) protein expressions

and investigate the effect of YHJD on TGF-β1-smad-ILK signal transduction pathway. Method: Unilateral ureteral obstruction (UUO) method was adopted to establish rat model of renal interstitial fibrosis

one hundred and thirty-two SD rats were randomly divided into normal group

sham operation group

model group

western medicine group

YHJD large

medium and low-dose group. Coomassie brilliant blue method was used to measure 24 h urinary protein. The protein expression of TGF-β1

smad7

p-smad2/3

ILK in kidney tissue were detected respectively by immunohistochemistry. Result: Compared with the normal group and sham operation group

TGF-β1

p-smad2

3

and ILK protein expressions in kidney tissue in he model group were significantly increased at 1

2

3 weeks (P<0.01)

there were weak expressions of TGF-β1

p-smad2/3

and ILK protein in normal group. TGF-β1

p-smad2/3

and ILK protein expressions in YHJD large

medium

low-dose groups were increased compared with those in the normal group and sham operation group at each time point. However

compared with the model group they were significantly reduced (P<0.01)

fosinopril sodium group was also significantly reduced than in model group (P<0.01). Conclusion: YHJD could block TGF-β1-smad-ILK signal transduction by reducing TGF-β1

p-smad2/3

ILK protein expressions and increasing smad7 proteins expression in kidney tissue. It might be the mechanisms of YHJD relieving renal interstitial fibroblast.