Influence of Drug-containing Serum Prepared for Jiedu Huayu Medicine on Expression of NF-B Pathway in Leukemia HL60/ADR Cell

MA Wu-kai; YAO Xue-ming; WANG Ying; HUANG Ying; HUANG Li-ming

doi:CNKI:11-3495/R.20110721.1732.002

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Influence of Drug-containing Serum Prepared for Jiedu Huayu Medicine on Expression of NF-B Pathway in Leukemia HL60/ADR Cell

更新时间：2024-01-04

- Influence of Drug-containing Serum Prepared for Jiedu Huayu Medicine on Expression of NF-B Pathway in Leukemia HL60/ADR Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 18, Pages: 154-157(2011)
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- DOI：CNKI:11-3495/R.20110721.1732.002
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- Published：2011
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MA Wu-kai, YAO Xue-ming, WANG Ying, et al. Influence of Drug-containing Serum Prepared for Jiedu Huayu Medicine on Expression of NF-B Pathway in Leukemia HL60/ADR Cell[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 154-157.
DOI：

MA Wu-kai, YAO Xue-ming, WANG Ying, et al. Influence of Drug-containing Serum Prepared for Jiedu Huayu Medicine on Expression of NF-B Pathway in Leukemia HL60/ADR Cell[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 154-157. DOI： CNKI:11-3495/R.20110721.1732.002.

摘要

目的: 探讨解毒化瘀药含药血清对白血病HL60/ADR细胞核周因子κB(NF-κB)信号基因表达的影响。方法: 将HL60/ADR耐药细胞分为牛血清组、兔血清组、含药血清高、中、低剂量处理组和干扰素对照组

用Western blot法检测各组细胞NF-κB/p65

NF-κB/p50

IκBα的表达情况。结果: 流式细胞仪检测结果显示非细胞毒作用的解毒化瘀药血清能够增加HL60/ADR细胞内柔红霉素的浓度。HL60/ADR耐药细胞NF-κB信号基因呈高表达

HL60敏感细胞未见表达

加用不同浓度解毒化瘀含药血清处理后

p65

p50和IKBa的表达均有不同程度下降

以高、中剂量组对p65的下调作用更明显。结论: 解毒化瘀药对HL60/ADR细胞的耐药逆转作用是通过阻断NF-κB信号通路实现的。

Abstract

Objective: To investigate the influence of drug-containing serum prepared for Chinese herbal medicine of Jiedu Huayu on the expression of nuclearfactor-κappaB(NF-κB)of leukemia HL60/ADR cell. Method: Western blot was applied for examination the expression of NF-κB/p65

NF-κB/p50

IκBα after HL60/ADR cell receiving the treatment of herbal serum of Jiedu Huayu. Result: The expression of NF-κB/p65

NF-κB/p50

IκBα after HL60/ADR cell was decreased by herbal serum of Jiedu Huayu. Conclusion: Jiedu Huayu Chinese herbal serum can reverse multidrug resistance in leukemia cell by blocking the expression of NF-κB pathway in leukemia HL60/ADR cell.

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