Objective: To explore attenuation that serum containing drug of Shenqi Fuzheng injection treatment on immunosuppression caused by chemotherapeutics. Method: Twenty male Wistar rates are divided into drug serum group and control group. Drug serum group is injected through caudal vein with 40 g ·kg-1 ·d-1 Shenqi Fuzheng injection twice a day and five times in total. Control group are injected with 0.9%NaCl solution instead of Shenqi Fuzheng injection and others are the same. All the rates’ blood are collected from abdominal aorta and then serum are Separated. Researches are divided into drug group and control group. In drug group

RAW264.7 cells are treated with Fluorouracil injection(50 mg ·L-1)

Cisplatin injection(6.25 mg ·L-1)

drug serum and in control group no-drug serum is used instead of drug. Treated RAW264.7 cells are detected with microplate reader.Observe effect on Fluorouracil injection

Cisplatin injection

serum containing drug of Shenqi Fuzheng injection on cell multiplication of RAW264.7 cells. Result: The serum containing drug of Shenqi Fuzheng injection can promote RAW264.7 cells multiplication(P<0.01). The serum containing drug of Shenqi Fuzheng injection can produce attenuation on immunosuppression caused by Fluorouracil injection and Cisplatin injection(P<0.01). Conclusions: In vitro environment Shenqi Fuzheng injection can promote RAW264.7 cells multiplication

and improve immunosuppression caused by chemotherapeutics. It can induce same trend and different intension of attenuation on different types of chemotherapy drugs.