Determination of Guanosine in Pinelliae Rhizoma by Capillary Zone Electrophoresis with Internal Standard Method

WEI Ying-qin; LU Heng; LIU Xin-xin; LIU Jin-xin; MENG Fan-yun; WANG Yong-yan

doi:CNKI:11-3495/R.20110721.1733.005

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Determination of Guanosine in Pinelliae Rhizoma by Capillary Zone Electrophoresis with Internal Standard Method

更新时间：2024-01-04

- Determination of Guanosine in Pinelliae Rhizoma by Capillary Zone Electrophoresis with Internal Standard Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 18, Pages: 49-51(2011)
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- DOI：CNKI:11-3495/R.20110721.1733.005
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- Published：2011
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WEI Ying-qin, LU Heng, LIU Xin-xin, et al. Determination of Guanosine in Pinelliae Rhizoma by Capillary Zone Electrophoresis with Internal Standard Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 49-51.
DOI：

WEI Ying-qin, LU Heng, LIU Xin-xin, et al. Determination of Guanosine in Pinelliae Rhizoma by Capillary Zone Electrophoresis with Internal Standard Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 49-51. DOI： CNKI:11-3495/R.20110721.1733.005.

摘要

目的: 建立毛细管区带电泳内标法测定半夏鸟苷含量的方法。方法: 采用毛细管区带电泳内标法定量

以熔融石英毛细管(57 cm×75 μm

有效长度50 cm)为分离通道

运行缓冲液经NaOH溶液调pH至9.0的0.8 mol ·L-1硼酸溶液-0.1 mol ·L-1硼砂溶液-乙腈(50∶50∶13)

分离电压25 kV

检测波长254 nm

毛细管温度25 ℃

自动压力进样5 s。结果: 半夏中鸟苷在0.25~1.5 mg线性关系良好(r=0.999 1)

鸟苷加样回收率为102.0%

RSD为2.4%。结论: 用毛细管区带电泳内标法测定半夏鸟苷含量的方法准确可行。

Abstract

Objective: To develop the capillary zone electrophoresis (CZE) method for quantitative analysis of guanosine in Pinelliae Rhizoma using aciclovir as internal standard. Method: A fused silica capillary (57 cm×75 μm ID

an effective length of 50 cm) was used.The running buffer was composed of 0.8 mol ·L-1 boric acid

0.1 mol ·L-1 borate and acetonitrile (50∶50∶13) with pH adjusted to 9.0.The applied voltage was 25 kV and the capillary temperature was 25 ℃. The detection wavelength was at 254 nm. Result: Good linearity was found in the range of 0.25-1.5 mg for guanosine with r=0.999 1.The average recovery was 102.0% for guanosine with the relative standard deviation 2.4% (n=5). Conclusion: The method is simple and accurate to determine guanosine in Pinelliae Rhizoma.

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