Objective: To observe effect of effective constituents of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma on the expression of α-SMA and E-cadherin in UUO rat kidney. Method: Sixty-six SD rats were randomly divided into 7 groups

normal group

model group

fosinopril group

salvianolic acids group

astragalus saponins group

granules combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group

constituents combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group. Each group was treated with different does of drugs after UUO surgery for 14 days. The doesage of fosinopril group was 6.6 mg·kg-1

salvianolic acids group was 40 mg·kg-1

astragalus saponins group was 37.5 mg·kg-1

constituents combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group was given salvianolic acids 40 mg·kg-1 and astragalus saponins group was 37.5 mg·kg-1

granules combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group was given Astragali Radix granules and Salviae Miltiorrhizae Radix et Rhizoma granules 10 g·kg-1.Blood was withdrawn from abdominal aortic to determine the renal function(creatinine

urea nitrogen)and metabolic cages were used to collect urine for retinol binding protein(RBP)determination

the obstructed kidney was taken out and fixed by 10% paraform and made into paraffin section by dehydration and embedding which were used for immunohistochemistry experiment. Result: The level of serum Cr in UUO rats reached (52.34±7.63) μmol·L-1

was higher than that of (43.29±4.13) μmol·L-1 in the normal group

however

except fosinopril

there was no significant difference between the treatment group and the model group No significant differences were found in the serum BUN. The standard of urinary RBP expression in every UUO group was significantly higher than normal group(P<0.05)

compared with the model group that its figure was(0.49±0.09)μmol·L-1

the fosinopril group and constituents group RBP levels were(0.41±0.05)

(0.41±0.04)μmol·L-1

of which had obvious significance(P<0.05). Immunohistochemical results showed that the most severely pathological changes was in the model group

the lightest pathological changes were found in fosinopril group and the group with constituents combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma. Conclusion: Renal function has been damaged in UUO rats

Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma effective constituents and their combination could ameliorate renal function and reduce the expression of tubular protein RBP in urine. Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma and their active constituents intervention could alleviate renal fibrosis at a certain degree.