LI Yan, CUI Xiang-ning, CHEN Ze-tao, et al. Effect of Blood-activating and Stasis-removing Herbs on Brain Water Content and Aquaporin4 Expression in Intracerebral Hemorrhage Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 153-156.
DOI:
LI Yan, CUI Xiang-ning, CHEN Ze-tao, et al. Effect of Blood-activating and Stasis-removing Herbs on Brain Water Content and Aquaporin4 Expression in Intracerebral Hemorrhage Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 153-156. DOI: CNKI:11-3495/R.20110809.1705.007.
Effect of Blood-activating and Stasis-removing Herbs on Brain Water Content and Aquaporin4 Expression in Intracerebral Hemorrhage Rats
Objective: To investigate the effects of blood-activating method on brain water content and aquaporin4(AQP4)expression in intracerebral hemorrhage(ICH)rats. Method: SD rats were randomly divided into the following groups:the sham operated group
model group
blood-activating high dose group and blood-activating low dose group. ICH rat model was established by injection autologous blood. The brain water content was determined by wet-dry weight
the expression of AQP4 protein and AQP4mRNA in cerebral tissues were determined respetively by immunohistochemitry techniques and real-time PCR. Result: Compared with those in the sham group
the expression of AQP4 mRNA and AQP4 protein increased significantly in the model group(P<0.01)
and the brain water content showed the same trend. Compared with the model group
the expression of AQP4 mRNA and AQP4 protein and the brain water content decreased obviously in blood-activating groups(P<0.01)
in a dose dependent manner. Conclusion: Blood-activating and stasis-removing herbs could decrease the edema of ICH
and the probable mechanism might be downregulation of AQP4.
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