TANG Yu-ping, JIANG Wei, SONG Shu-lin, et al. Dose-response Relationship of Free Radical Scavenging Activity by Blood-activating and Stasis-removing Formulae[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 142-145.
DOI:
TANG Yu-ping, JIANG Wei, SONG Shu-lin, et al. Dose-response Relationship of Free Radical Scavenging Activity by Blood-activating and Stasis-removing Formulae[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 142-145. DOI: CNKI:11-3495/R.20110809.1705.010.
Dose-response Relationship of Free Radical Scavenging Activity by Blood-activating and Stasis-removing Formulae
Objective: To study the effects of the free radical scavenging activity of these formulae for blood-activating and stasis-removing such as Xuefu Zhuyu Decoction(XF)
Shaofu Zhuyu Decoction(SF)
Gexia Zhuyu Decoction(GX)
Shentong Zhuyu Decoction(ST)and Tongqiao Huoxue Decoction(TQ). Method: Free radical scavenging activity was determined by 2
2-diphenyl-1-picrylhydrazyl(DPPH)method. Result: The result showed that all these formulae had significant effects on scavenging DPPH radical(P<0.01)
in a dose-dependent manner.. The free radical scavenging capacity on DPPH were in the following descending order:GX>TQ>SF>ST>XF. The scavenging ability in total formula was better than in ethanol precipitant and supernatant. Conclusion: Validation studies proved that the enzyme-linked immunosorbent assay for DPPH had good reproducibility and feasibility. The method could be used as high throughput screening for Chinese herbs and traditional chinese medicine formulae.
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Related Author
Sheng-jie HUANG
Qi-qi HU
Peng TAN
Hua-mei GOU
Xi-chuan WEI
Hao-zhou HUANG
San-hu FAN
Jun-zhi LIN
Related Institution
School of Pharmacy,State Key Laboratory of Characteristic Chinese Drug Resources in Southwest China,Chengdu University of Traditional Chinese Medicine(TCM)
State Key Laboratory of Biological Evaluation of TCM Quality,National Administration of TCM, Sichuan Academy of Chinese Medicine Sciences
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Teaching Hospital of Chengdu University of TCM
College of Chemistry,Chemical Engineering and Environment,Qingdao University