Effects of Tanshinone Ⅱ on Renal Nephrin and Transforming Growth Factor- of Adriamycin Nephritic Rats

WANG Jun-jian; HU-Rui

doi:CNKI:11-3495/R.20110809.1705.011

您当前的位置：

首页 >

文章列表页 >

Effects of Tanshinone Ⅱ on Renal Nephrin and Transforming Growth Factor- of Adriamycin Nephritic Rats

更新时间：2024-01-04

- Effects of Tanshinone Ⅱ on Renal Nephrin and Transforming Growth Factor- of Adriamycin Nephritic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 19, Pages: 245-251(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：CNKI:11-3495/R.20110809.1705.011
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
WANG Jun-jian, HU-Rui. Effects of Tanshinone Ⅱ on Renal Nephrin and Transforming Growth Factor- of Adriamycin Nephritic Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 245-251.
DOI：

WANG Jun-jian, HU-Rui. Effects of Tanshinone Ⅱ on Renal Nephrin and Transforming Growth Factor- of Adriamycin Nephritic Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 245-251. DOI： CNKI:11-3495/R.20110809.1705.011.

摘要

目的: 探讨肾小球足细胞跨膜蛋白(nephrin)、转化生长因子-β1(transforming growth factor-β1

TGF-β1)在阿霉素肾病大鼠肾小球中的表达变化及丹参酮 ⅡA对其影响。方法: SD大鼠56只随机分为2组

模型组44只和对照组12只。模型组大鼠尾静脉一次注射盐酸阿霉素(ADR)0.007 g·kg-1

对照组大鼠尾静脉注射等容积生理盐水

注射ADR 1周后尿蛋白定量>100 mg·(24 h)-1即算建模成功

共有40只大鼠建模成功

随机又分为肾病模型组、泼尼松组、丹参酮ⅡA低剂量组和高剂量组

每组10只。泼尼松组给予泼尼松0.01 g·kg-1·d-1 ig;丹参酮ⅡA低剂量组和高剂量组分别给予丹参酮ⅡA 0.02

0.04 g·kg-1·d-1 ig

共4周。对照组和肾病模型组给予等容积生理盐水ig。实验结束后检测各组大鼠血、尿生化指标

光镜观察肾脏病理学改变

免疫组化技术和Western blotting技术检测nephrin和TGF-β1的表达。结果: 肾病模型组、泼尼松组、丹参酮ⅡA低剂量组和高剂量组24 h尿蛋白定量、胆固醇、甘油三酯、血尿素氮、血肌酐和肾小球TGF-β1表达均高于对照组(P<0.05)

但泼尼松组和丹参酮ⅡA高剂量组上述指标均低于肾病模型组(P<0.05);肾病模型组、泼尼松组、丹参酮ⅡA低剂量组和高剂量组血清总蛋白、白蛋白、肾小球nephrin表达低于对照组(P<0.05)

但泼尼松组和丹参酮 IIA高剂量组血清总蛋白、白蛋白、nephrin表达高于肾病模型组(P<0.05)。结论: Nephrin及TGF-β1 在阿霉素肾病发病机制中起重要作用;丹参酮ⅡA能够增加肾组织nephrin的表达

抑制TGF-β1的表达

减少阿霉素肾病大鼠尿蛋白的排出

减轻及延缓肾小球硬化

发挥保护肾脏的作用。

Abstract

Objective: To explore effects of tanshinone ⅡA on expression changes of nephrin and transforming growth factor-β1(TGF-β1)in adriamycin(ADR)-induced-nephropathy rats. Method: Fifty-six rats of Sprague-Dawley were divided into two groups:the model group(n=44)and the control group(n=12).The rats in the model group were injected with a single dose of ADR(0.007 g·kg-1)via tail-vein

while the rats in the control group were injected with a comparable volume of 0.9% saline. The model was successfully established when the 24 h urinary protein excretion exceeded 100 mg after ADR injection 1 week

forty rats were successful and then were randomly assigned to the ADR nephrosis group(n=10)

prednisone group(n=10)

the low dose and high dose of tanshinone ⅡA group(n=10). The rats in the prednisone group were treated with prednisone(0.01 g·kg-1·d-1)

The rats in the low dose and high dose of tanshinone ⅡA group were treated with tanshinone ⅡA(0.02 g·kg-1·d-1 or 0.04 g·kg-1·d-1)

once a day for 4 weeks

whereas the other rats were received a comparable volume of 0.9% saline. The blood and urina biochemical indicators were measured and the pathological changes of the renal tissues were observed under light microscope

immunohistochemical and Western blotting techniques to detect the expression of nephrin and TGF-β1. Result: The 24 h urinary protein

cholesterol

triglyceride

blood urea nitrogen

serum creatinine

and the expression of TGF-β1 in the ADR nephrosis group

prednisone group

the low dose and high dose of tanshinone ⅡA group were higher than those in the control group(P<0.05). The total protein

albumin and the expression of nephrin in the ADR nephrosis group

prednisone group

the low dose and high dose of tanshinone ⅡA group was lower than those in the control group(P<0.05). After treatment with prednisone and the high dose of tanshinone ⅡA could improve above-mentioned indicators. Conclusion: Nephrin and TGF-β1 play an important role in the pathogenesis of ADR nephrosis; tanshinone ⅡA decreased the urine albumin excretory rate

and prevent the glomemlar sclerosis in rats with ADR nephrosis

which mechanism may be at least partly correlated with enhancing the expression of nephrin and inhibiting the expression of TGF-β1 in kidney.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective Effect of Arctinin Capsule on the Nephrotic Rat Induced by Adriamycin

Clinical Efficacy of Modified Huangqi Chifengtang in Treatment of IgA Nephropathy Patients and Exploration of Dose-effect Relationship of Astragali Radix

Anti-inflammatory Mechanism of Wuwei Xiaoduyin on IgA Nephropathy Rats Based on NF-κB Inflammatory Signaling Pathway

Huidouba Ameliorates Kidney Oxidative Stress Injury by Down-regulating Nox4 Expression in Rats with Diabetic Nephropathy

Treatment of Renal Proteinuria by Traditional Chinese Medicine and Its Mechanism

Related Author

LI Jun

DONG Xin-xin

LUO Ying-ying

LIANG Bin

CHEN Lan-ying

SHI Xiujie

CHANG Meiying

SHI Yue

Related Institution

Beijing University of Chinese Medicine

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Hebei Hospital of Traditional Chinese Medicine

Hebei University of Chinese Medicine

Chengde Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰