Chemical Constituents of Tongmai Formula

WANG Fu-rong; GE Xi-zhen; YANG Xiu-wei

doi:CNKI:11-3495/R.20110823.1118.010

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Chemical Constituents of Tongmai Formula

更新时间：2024-01-04

- Chemical Constituents of Tongmai Formula
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 20, Pages: 61-69(2011)
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- DOI：CNKI:11-3495/R.20110823.1118.010
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- Published：2011
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WANG Fu-rong, GE Xi-zhen, YANG Xiu-wei. Chemical Constituents of Tongmai Formula[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 61-69.
DOI：

WANG Fu-rong, GE Xi-zhen, YANG Xiu-wei. Chemical Constituents of Tongmai Formula[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 61-69. DOI： CNKI:11-3495/R.20110823.1118.010.

摘要

目的: 研究通脉方(葛根、丹参、川芎)的化学成分

为中药复方物质基础研究提供示范。方法: 采用大孔吸附树脂、硅胶、Sephadex LH-20、反相高效液相色谱等柱色谱法进行分离、纯化

根据化合物的理化性质和谱学数据鉴定其结构。结果: 从通脉方水提取物中分离得到34个化合物

分别鉴定为:丹参酮I(1)、隐丹参酮(2)、二氢丹参酮I(3)、芒柄花素(4)、原儿茶醛(5)、大豆苷元(6)、反式-阿魏酸(7)、芒柄花苷(8)、5-羟基芒柄花苷(9)、洋川芎内酯H(10)、葛根苷D(11)、染料木苷(12)、大豆苷(13)、3'-甲氧基葛根素(14)、芒柄花素-8-C-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(15)、芒柄花素-7-O-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(16)、澳白檀苷(17)、5

7-二羟基-4'-甲氧基异黄酮-7-O-β-D-木糖基-(1→6)-β-D-吡喃葡萄糖苷(18)、鹰嘴豆芽素 A-8-C-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(19)、葛根素(20)、蔗糖(21)、腺嘌呤核苷(22)、葛根素芹菜糖苷(23)、3'-羟基葛根素(24)、3'-甲氧基大豆苷元-7

4'-二-O-β-D-吡喃葡萄糖苷(25)、大豆苷元-7

4'-O-葡萄糖苷(26)、3'-甲氧基大豆苷(27)、芒柄花素-8-C-β-D-吡喃木糖基-(1→6)-O-β-D-吡喃葡萄糖苷(28)、染料木素-7-O-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(29)、染料木素-8-C-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(30)、大豆苷元-7-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃葡萄糖苷(31)、大豆苷元-7-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃葡萄糖苷(32)、3'-羟基葛根素芹菜糖苷(33)、6″-O-D-木糖基葛根素(34)。结论: 综合以上及葛根、丹参、川芎单味药材化学成分的研究结果

判断通脉方主要化学成分为异黄酮类

来源于组方中的葛根;3个丹参酮类化合物来源于组方中的丹参;洋川芎内酯H来源于组方中的川芎。虽然单味药材的化学成分在复方中皆有体现

但主要是君药葛根的化学成分。16

29为首次从葛根及葛属中分离得到。

Abstract

Objective: To study the chemical constituents of Tongmai formula and set an example for its bioactive substances foundation investigation. Method: The chemical constituents were isolated and purified by column chromatography of macroporous resin

silica gel

Sephadex LH-20 and reversed phase high-performance liquid chromatography

and their structures were identified by physico-chemical properties and spectrum analysis. Result: Thirty-four compounds were separated and identified as tanshinone I (1)

cryptotanshinone (2)

dihydrotanshinone I (3)

formononetin (4)

protocatechuic aldehyde (5)

daidzein (6)

trans-ferulic acid (7)

ononin (8)

5-hydroxylononin (9)

senkyunolide H (10)

(4R)-3- -4-(4-β-D-glucopyranosyloxybenzyl)-but-2-en-4-olide (pueroside D

11)

genistin (12)

daidzin (13)

3'-methoxypuerarin (14)

formononetin-8-C-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (15)

formononetin-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (16)

lanceolarin (17)

kakkanin (18)

biochanin A-8-C-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (19)

puerarin (20)

sucrose (21)

adenosine (22)

mirificin (23)

3'-hydroxypuerarin (24)

3'-methoxydaidzein-7

4'-di-O-β-D-glucopyranoside (25)

daidzein-7

4'-O-glucoside (26)

3'-methoxydaidzin (27)

formononetin-8-C-β-D-xylopyranosyl-(1→6)-O-β-D-glucopyranoside (28)

genistein-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (ambocin

29)

genistein-8-C-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (30)

daidzein-7-O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranoside(31)

daidzein-7-O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranoside(32)

3'-hydroxymirificin (33)

6″-O-D-xylosylpuerarin (34). Conclusion: On the basis of the above mentioned results and previously reported chemical studies on the Puerariae Lobatae Radix

Salviae Miltiorrhizae Radix et Rhizoma

and Chuanxiong Rhizoma

it was suggested that the main chemical components were isoflavanoid compounds rised from the Puerariae Lobatae Radix

and the secondary components were tanshinones rised from the Salviae Miltiorrhizae Radix et Rhizoma

and senkyunolide H rised from the Chuanxiong Rhizoma in the Tongmai formula. Compaunds 16

29 were isolated from Puerariae Lobatae Radix and the genus Pueraria DC. for the first time.

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Related Institution

Key Laboratory of Chinese Materia Medica, Key Laboratory of Basic and Application Research of Northern Medicine under Ministry of Education, Heilongjiang Key Laboratory of Traditional Chinese Medicine(TCM) and Natural Medicine Pharmacodynamic Material Bases, Heilongjiang University of Chinese Medicine

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