LIU Dong, DU Shou-ying, HE Xiu-feng, et al. Studies on Simultaneous Determination and Pharmacokinetics of Two Bufadienolides in Rabbits Plasma[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 135-138.
DOI:
LIU Dong, DU Shou-ying, HE Xiu-feng, et al. Studies on Simultaneous Determination and Pharmacokinetics of Two Bufadienolides in Rabbits Plasma[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 135-138. DOI: CNKI:11-3495/R.20110906.1107.004.
Studies on Simultaneous Determination and Pharmacokinetics of Two Bufadienolides in Rabbits Plasma
Objective: To establish a HPLC method for the assay of bufalin (BL)
resibufogenin (RBG) and study the pharmacokinetics of BL
RBG in rabbits plasma. Method: The sample blood from rabbits were injected venenum bufonis extract by 0.35 mg·kg-1 at 2
5
10
15
20
30
45
60
90 min. Protein precipitation with acetonitrile and liquid-liquid extraction was applied to purify plasma samples and used HPLC to determine the concentration of BL
RBG in it. The pharmacokinetics parameters were accessed by Kinetica software. Result: In this HPLC method
the separation
precision and accuracy of BL
RBG were well. The calibration curves were linear. The analytical recoveries were all more than 90%
intra and inter-day precision RSD were all less than 15.0% ( n =5). The main pharmacokinetics parameters were fitted by non-compartment models. Conclusion: The HPLC method can be used to determine the concentration and to investigate the pharmacokinetics of BL and RBG.
Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs
Comparison of Pharmacokinetics and Tissue Distribution of Free Anthraquinones and Gallic Acid in Mice with Ulcerative Colitis Before and After Processing of Rhei Radix et Rhizoma Carbonisata
Pharmacokinetic Differences of Seven Components in Different Phases of Banxia Xiexintang in Rats
Pharmacological Mechanism of Huanglian Jiedutang: A Review
Pharmacokinetic Analysis of Ziyuglycoside Ⅰ in Normal and Acute Kidney Injury Rats
Related Author
HE Xiu-feng
DU Shou-ying
DAI Chun-hong
GU Xue-zhu
ZHAO Li-min
SUN Jing
QI Yunpeng
LI Siyuan
Related Institution
School of Life Sciences,Beijing University of Chinese Medicine
School of Pharmacy,Shanghai University of Traditional Chinese Medicine
First Teaching Hospital of Tianjin University of TCM
The Second Affiliated Hospital of Tianjin University of TCM
School of Graduate Studies,Tianjin University of Traditional Chinese Medicine(TCM)