Objective: To study the components of the essential oil from the leaves of Lindera obtusiloba and the antimicrobial intensity. Method: Distillation in water vapor was applied to extract the essential oil from the leaves of L. obtusiloba and meanwhile gas chromatography-mass spectrometry(GC-MS) was used to analyze the components of this plant. Meanwhile

paper disc diffusion method and plate serial dilution method were used respectively to study the anti-bacterial activity and the minimum inhibitory concentration(MIC). Result: The essential oil was extracted successfully and 46 compounds were identified. Also broad-spectrum antimicrobial activity was found. Conclusion: Main components of the essential oil from the leaves of L. obtusiloba are alcohol

sesquiterpenes

and a minority of esters

oxygenated components as well as some other compounds. And the antimicrobial intensity to the tested strains is: Staphylococcus aureus > Staphylococcus epidermidis > Enterococcus faecium > Escherichia coil > Penicillium citrinum > Staphylococcus albus > Bacillus subtilis > Candida albicans>Aspergillus niger > Pseudornonas aeruginosa.