Study on Metabolites of Gegen Qinlian Dispensing Granule in Rat

CHEN Li-hong; FAN Hui-jia; TANG Yu-ping; BIAN Yong; YANG Nian-yun; JIN Shan-lin; WANG Qiang

doi:CNKI:11-3495/R.20111017.0939.001

您当前的位置：

首页 >

文章列表页 >

Study on Metabolites of Gegen Qinlian Dispensing Granule in Rat

更新时间：2024-01-04

- Study on Metabolites of Gegen Qinlian Dispensing Granule in Rat
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 24, Pages: 101-104(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：CNKI:11-3495/R.20111017.0939.001
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
CHEN Li-hong, FAN Hui-jia, TANG Yu-ping, et al. Study on Metabolites of Gegen Qinlian Dispensing Granule in Rat[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 101-104.
DOI：

CHEN Li-hong, FAN Hui-jia, TANG Yu-ping, et al. Study on Metabolites of Gegen Qinlian Dispensing Granule in Rat[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 101-104. DOI： CNKI:11-3495/R.20111017.0939.001.

摘要

目的 : 研究葛根芩连配方颗粒在大鼠尿液中的代谢产物化学结构。方法 :采用HPLC-DAD-MS技术

选用Lichrospher C18色谱柱(4.6 mm×150 mm

5 μm)

流动相0.1%甲酸水 (A)-乙腈 ∶甲醇(6 ∶4)(B)

检测波长272 nm; ESI离子源

正、负离子模式。结果 :从灌胃葛根芩连配方颗粒的大鼠尿液中

发现并鉴定了11 个主要代谢产物的化学结构

其中有5个来自葛根药材

分别为葛根素、3'-甲氧基葛根素、7 -木糖苷葛根素、葛根素-葡萄糖苷、大豆苷元-4'-O-硫酸酯;有4个来自黄芩药材

分别为野黄芩素-6-O-葡萄糖苷、汉黄芩素、千层纸素A;有2个来自甘草药材

分别为异甘草素、甘草素。结论 : 葛根芩连配方颗粒在大鼠尿液中代谢产物主要以苷元或以葡萄糖醛酸、硫酸的结合物排泄

一些成分如葛根素主要以原形排泄。

Abstract

Objective :To study the metabolism of Gegen Qinlian dispensing granule in rat. Method : The urine samples containing metabolites of Gegen Qinlian dispensing granule were analyzed by HPLC-DAD-MS. Chromatographic systemwas composed of lichrospher C18 column (4.6 mm×250 mm

5 μm)

0.1% formic acid (A)-acetonitrile ∶methanol(6 ∶4)(B) as the mobile phase

detection at 272 nm; ESI source

positive and neglative mode. Result: Eleven components were identified as puerarin -glucoside

scutellarein-6-O-glucoside

puerarin

3' -methoxypuerarin

7-xylosidepuerarin

daidzein-4'-O-sulfate

Baicalein-6

7-O-diglucuronide

isoliquiritigenin

liquiritigenin

wogonin

oroxylin A. Conclusion: Metabolites of Gegen Qinlian dispensing granule in rat were conjugated to glycuronic acid and sulphuric acid or excreted as aglycone

some components such as puerarin were mainly excreted unchanged in the urine.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Comparative Analysis of Serum Pharmacochemistry of Alismatis Rhizoma Before and After Salt Processing Based on UPLC-Q-TOF-MS

Determination of Glycyrrhizin in Weiwanshu Granule by HPLC

Determination of Icariin in Syrup by HPLC

双黄连含片中黄芩甙、绿原酸及连翘甙的含量测定

Determination of Puerarin in Gegen Qinlian Weiwan by HPLC

Related Author

YAN Lin

OU Zemin

WANG Yanjing

ZHANG Yao

CHENG Yi

WANG Zicheng

LIU Dewen

WANG Jinyu

Related Institution

Beijing City University

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Jiangxi University of Chinese Medicine

广西中医药大学赛恩斯新医药学院,广西中医药大学,广西中医药大学赛恩斯新医药学院

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰