Objective : To investigate the protective effect on cardiomyocyts against anoxia/reoxygenation(A/R) injury in Guanxin Danshen compounding of the best ratio

if lignum acronylchiae oil and/or lignum acronylchiae total flavonoids was removed

or tanshinone from salvia miltiorrhiza was added

of the contribution of various components on the efficacy. Method : Taken cultured neonatal rat ventricular cardiomyocytes for 4 days were randomly divided into seven groups: Control group; (1) A/R group; (2) 1% DSMO group; (3) the group treated with the best ratio; (4)-(6): these groups respectively treated with lignum acronylchiae oil and/or lignum negative total flavonoids

or salviol of different dose 1

2

4 mg added; cell viability

superoxide dismutase(SOD)

Catalase

glutathione peroxidase(GSH-Px) activities

malondialdehyde(MDA) contents and lactate dehydrogenase(LDH) activitied were detected. Result : In Guanxin Danshen compounding of the best ratio

the protective effect of cardiomyocyte against A/R injury was significantly decreaed if lignum acronylchiae oil and/or lignum acronylchiae total flavonoids was removed

these changes of anti-lipid oxidation indexes such as SOD

Catalase

GSH-Px activities and MDA contents were more obvious than other indexes; Once salviol was added

the protective effect on cardiomyocyte against A/R was enchanced with different degree

espcially LDH and cell viability was obvious and 2 mg salviol was the best protective effect. Conclusion : In the Guanxin Danshen compounding of the best ratio

lignum acronylchiae oil and lignum acronylchiae total flavonoids were necessary; Adding 2 mg salviol could enchance the protective effect on cardiomyocyte.