Effects of Suoquan Wan on -ARmRNA Expression of Detrusor in Natural Aging Rats

CAO Hong-yin; WU Jun; WU Qing-he; HUANG Ping

doi:CNKI:11-3495/R.20111107.1625.005

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Effects of Suoquan Wan on -ARmRNA Expression of Detrusor in Natural Aging Rats

更新时间：2024-01-04

- Effects of Suoquan Wan on -ARmRNA Expression of Detrusor in Natural Aging Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 1, Pages: 125-128(2012)
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- DOI：CNKI:11-3495/R.20111107.1625.005
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- Published：2012
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CAO Hong-yin, WU Jun, WU Qing-he, et al. Effects of Suoquan Wan on -ARmRNA Expression of Detrusor in Natural Aging Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 125-128.
DOI：

CAO Hong-yin, WU Jun, WU Qing-he, et al. Effects of Suoquan Wan on -ARmRNA Expression of Detrusor in Natural Aging Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 125-128. DOI： CNKI:11-3495/R.20111107.1625.005.

摘要

目的: 观察自然衰老大鼠逼尿肌β3-肾上腺素能受体(AR)mRNA表达的改变

探讨缩泉丸"缩尿"的机制。方法: 取老年大鼠(20月龄)50只

青年大鼠(3月龄)10只。设置组别为:空白对照组、模型对照组、金匮肾气丸组(1.08 g ·kg-1 ·d-1)、缩泉丸高、中、低剂量组(0.293

0.585

1.170 g ·kg-1 ·d-1)

每组10只。空白对照组和模型对照组给予同体积蒸馏水

缩泉丸低、中、高剂量组给予相应浓度的缩泉丸药液

金匮肾气丸组予金匮肾气丸药液

连续4周。选用代谢笼法测量大鼠24 h尿量及水负荷5 h尿量

采用PCR方法检测自然衰老大鼠逼尿肌β3-AR mRNA表达。结果: 缩泉丸中、高剂量组动24 h尿量明显减少

分别为(4.18±2.07)

(4.16±1.54)mL ·(100g)-1;缩泉丸低、中、高剂量组水负荷后5 h内尿量明显减少

分别为(1.08±0.43)

(0.99±0.54)

(0.96±0.49)mL ·(100g)-1;自然衰老大鼠逼尿肌β3-AR mRNA表达明显下降(P<0.05)

相对比值为1.61±0.045;给予缩泉丸后

中、高剂量可以显著提高自然衰老大鼠膀胱逼尿肌中β3-AR mRNA的表达(P<0.05)

相对比值为1.63±0.036

1.70±0.061。结论: 缩泉丸可以上调自然衰老大鼠逼尿肌β3-ARmRNA的表达

可能是其发挥"缩尿"作用的机制。

Abstract

Objective: The mechanism of Suoquan Wan(SQW) reducing urination was investigated by observing the change of the expression of β3- adrenergic receptor(AR)of mRNA in bladder detrusor of the natural aging rat. Method: Fifty aged rats (20 months) and 10 young rats (3 months) were chosen. Young rats were chosen as control

aged rats were randomly divided into five groups: model

Jinkui Shenqi pill group

SQW high

middle and low dose group

10 rats in each group. The low

middle and high dose group of SQW were given the appropriate concentration (0.293

0.585

1.170 g ·kg-1 ·d-1). Jinkui Shenqi pill group was given appropriate liquid(1.08 g ·kg-1 ·d-1)

once everyday for four weeks. The urination after the water load in rats at 5 h and 24 h was measured in metabolic cages.β3-AR mRNA expression was detected by PCR in the detrusor of the natural aging rats. Result : The 24 h urination of SQW middle and high dose group significantly decreased. The 5h urination after the water load in rats of SQW low

middle and high dose group also significantly decreased.β3-AR mRNA expression significantly decreased in the detrusor of the natural aging rats(P<0.05). SQW high dose can remarkably elevate β3-AR mRNA expression in the detrusor of the natural aging rats after administration SQW. Conclusion : SQW can remarkably elevate β3-AR mRNA expression in the detrusor of the natural aging rats

which was the possible mechanism of SQW reducing urination.

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