Objective: To study the effect of 6-BA

naphthyl acetic acid(NAA)

carbon source and pH on the cell suspension culture

and to explore the optimal conditions for establishing the cell suspension culture system. Method: The fresh weight and dry weight were investigated in a cell cycle

the growth curve and growth rate curves were drawn. The combination of 4 factors and 3 levels of 6-BA

NAA

carbon source and pH were optimized by orthogonal test method. Result: The growth cycle of Siraitia grosvenorii cell suspension culture was 21 days

the lag phase was 0-6 d

the log phase was 7-21 d

there was transient stability in the stable phase

the decline phase achieved on the 22th day. The highest fresh weight and dry weight were achieved on the 21th day

and reached 693.0 g·L-1and 416.0 g·L-1 respectively

the maximum growth rate reached 0.040 g·L-1 ·d-1. The NAA level was the key factor of establishing the cell suspension culture system of S. grosvenorii

the effect of the four factors was NAA>6-BA>pH>carbon source

the optimal level was MS+1.0 mg·L-1 6-BA+0.02 mg·L-1 NAA

4.0% sucrose and pH 5.5-6.0 initial medium. Conclusion: The cell suspension culture system of S. grosvenorii can be established successfully by tissue culture.