Effects of Qi Supplement and Blood Activation Prescription and its Disassembled Prescriptions Medicated Serum on the Secretion of MMP-9, MMP-2 by Human Umbilical Vein Endothelial Cell Post-transfected VEGF

CHEN Meng; LOU Li-xia; WU Ai-ming; CHAI Li-min; LV Xi-ying; ZHANG Dong-mei

doi:CNKI:11-3495/R.20111116.1424.007

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Effects of Qi Supplement and Blood Activation Prescription and its Disassembled Prescriptions Medicated Serum on the Secretion of MMP-9, MMP-2 by Human Umbilical Vein Endothelial Cell Post-transfected VEGF

更新时间：2024-01-04

- Effects of Qi Supplement and Blood Activation Prescription and its Disassembled Prescriptions Medicated Serum on the Secretion of MMP-9, MMP-2 by Human Umbilical Vein Endothelial Cell Post-transfected VEGF
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 2, Pages: 156-160(2012)
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- DOI：CNKI:11-3495/R.20111116.1424.007
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- Published：2012
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CHEN Meng, LOU Li-xia, WU Ai-ming, et al. Effects of Qi Supplement and Blood Activation Prescription and its Disassembled Prescriptions Medicated Serum on the Secretion of MMP-9, MMP-2 by Human Umbilical Vein Endothelial Cell Post-transfected VEGF[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 156-160.
DOI：

CHEN Meng, LOU Li-xia, WU Ai-ming, et al. Effects of Qi Supplement and Blood Activation Prescription and its Disassembled Prescriptions Medicated Serum on the Secretion of MMP-9, MMP-2 by Human Umbilical Vein Endothelial Cell Post-transfected VEGF[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 156-160. DOI： CNKI:11-3495/R.20111116.1424.007.

摘要

目的: 探讨活血益气方及其拆方含药血清对含有血管内皮生长因子(VEGF)165的重组质粒pcDNA3.1-VEGF165转染人脐静脉内皮细胞(HUVEC)分泌基质金属蛋白酶(MMP)2

9的影响。方法: 构建pcDNA3.1-VEGF165重组质粒

采用Fugene HD将质粒瞬时转染至HUVEC中。制备活血益气方及其拆方含药大鼠血清、生理盐水正常血清

以5%药物血清作用于转染后的HUVEC

Western blot法检测VEGF的表达

ELISA法测定MMP-2

MMP-9的表达。结果: (1)活血益气方组VEGF蛋白表达高于生理盐水组

与之比较有统计学差异(P<0.01)。拆方各组则低于活血益气方组

与之比较有统计学差异(P<0.01)。(2)活血益气方组、活血方组MMP-2含量高于生理盐水组

与之比较有统计学差异(P<0.05

P<0.01)。拆方各组中仅益气方组MMP-2含量低于活血益气方组

与之比较有统计学差异(P<0.01)。(3)活血益气方及其拆方各组MMP-9含量均高于生理盐水组

仅活血益气方组、活血方组与之比较有统计学差异(P<0.01

P<0.05)。拆方各组MMP-9含量均低于活血益气方组

与之比较具有统计学差异(P<0.01)。结论: 活血益气方可以促进转染后HUVEC表达VEGF及分泌MMP-2

MMP-9

活血方药在这方面起主要作用。推测其治疗性血管生成作用机制可能与促进内皮细胞分泌MMP

从而促进内皮细胞迁移

提高血管通透性有关。

Abstract

Objective: To observe the effects of Qi supplement and blood activation prescription (QB)and its disassembled prescriptions medicated serum on the secretion of matrix metalloproteinase (MMP) 9 and MMP-2 by human umbilical vein endothelial cells (HUVEC) post-transfected pcDNA3.1-VEGF165. Method: Constructing pcDNA3.1-VEGF165 restructuring plasmid

then it was transient transfected in HUVEC with Fugene HD transfection reagent. Prepare QB and its disassembled prescriptions medicated rat serum

saline normal serum. Seed the post-transfected HUVEC with 5% serum. The expression of VEGF was detected by using Western Blot. The expression of MMP-2 and MMP-9 were detected by using enzyme-linked immunosorbent assay. Result: (1)The expression of vascular endothelial growth factor(VEGF) in the QB group is higher than the saline group. There is a significant difference between the two groups(P<0.01). The expression of VEGF in the Blood Activation (BA) group and the Qi supplement (QS) group are lower than the QB group. Both groups have statistically significant difference when compare with the whole group(P<0.01). (2)Either the secretion of MMP-2 in the QB group or in the BA group is higher than the saline group. Both groups have statistically significant difference when compare with the Saline group(P<0.05

P<0.01). The secretion of MMP-2 in the QS group is lower than the QB group. There is a significant difference between the two groups(P<0.01). (3)The secretion of MMP-9 in the QB group and its disassembled prescriptions group are higher than the Saline group. Only the whole group and the BA group have significant with it(P<0.01

P<0.05). The secretion of MMP-9 in the disassembled prescriptions groups is lower than the whole group. Both groups have significant with the whole group(P<0.01). Conclusion: QB can not only promote the expression of VEGF

but also increase the secretion of MMP-9 and MMP-2. BA plays a main role in this. The therapeutic angiogenesis mechanisms of QB may be related to increase the secretion of MMP

accordingly promote endothelial cell migration

and improve vascular permeability.

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