Study on the Treatment of Tripterginum Wilfordii Polyglycoside Combined with Leflunomide on Rat Adjuvant Arthritis and its Mechanisms

QU Fei; CUI Yan-ru; XU Jing

doi:CNKI:11-3495/R.20111202.1023.001

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Study on the Treatment of Tripterginum Wilfordii Polyglycoside Combined with Leflunomide on Rat Adjuvant Arthritis and its Mechanisms

更新时间：2024-01-04

- Study on the Treatment of Tripterginum Wilfordii Polyglycoside Combined with Leflunomide on Rat Adjuvant Arthritis and its Mechanisms
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 3, Pages: 128-131(2012)
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- DOI：CNKI:11-3495/R.20111202.1023.001
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- Published：2012
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QU Fei, CUI Yan-ru, XU Jing. Study on the Treatment of Tripterginum Wilfordii Polyglycoside Combined with Leflunomide on Rat Adjuvant Arthritis and its Mechanisms[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 128-131.
DOI：

QU Fei, CUI Yan-ru, XU Jing. Study on the Treatment of Tripterginum Wilfordii Polyglycoside Combined with Leflunomide on Rat Adjuvant Arthritis and its Mechanisms[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 128-131. DOI： CNKI:11-3495/R.20111202.1023.001.

摘要

目的: 研究雷公藤多苷联合来氟米特普配伍使用对佐剂性关节炎(AA)大鼠的治疗效果及其可能的作用机制。方法: 建立AA大鼠关节炎模型

分为对照组、模型组、来氟米特组、雷公藤多苷组和雷公藤多苷联合来氟米特配伍组;来氟米特20 mg ·kg-1 ig

1次/d;雷公藤多苷组:雷公藤多苷20 mg ·kg-1 ig

1次/d;配伍组:来氟米特20 mg ·kg-1 ig

8 h后

雷公藤多苷20 mg ·kg-1 ig

1次/d均于造模后14 d开始给药

连续处理21 d。治疗后

观察AA大鼠足爪肿胀程度及关节炎指数(AI)

采用放射免疫法检测血清中白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达水平

酶联免疫吸附法(ELISA)检测血清中干扰素-γ(IFN-γ)、IL-4和前列腺素E2(PGE2)含量。结果: ① 相较于AA模型组

来氟米特组、雷公藤多苷组和配伍组中大鼠足爪肿胀程度及AI评分均显著下降(P均<0.01)

其中以配伍组大鼠关节炎症状改善最为明显(P<0.05)。② 模型组大鼠血清IL-1β

TNF-α

IL-6和PGE2水平明显高于正常对照组(P<0.01)

而经过治疗后血清中各细胞因子含量均发生不同程度的下降

且以配伍组降低最为显著(P<0.01或P<0.05);③ 模型组大鼠血清中IFN-γ表达水平明显高于正常对照组

而IL-4含量明显低于对照组(P<0.01)

治疗后各组中AA大鼠血清中IFN-γ水平显著降低

而IL-4水平明显升高(P<0.05)

其中以配伍组变化最为明显(P<0.01)。结论: 雷公藤多苷和来氟米特联合使用可显著降低血清中炎症因子水平

减少关节破坏

减轻AA大鼠的关节炎症状。

Abstract

Objective: To study the therapeutic effects and possible mechanism of tripterginum wilfordii polyglycoside (TWP) combined with leflunomide (LEF) on rat adjuvant arthritis (AA). Method: The murine collagen-induced arthritis model was established

then the arthritic rats were divided into control group

AA model group

LEF group

TWP group and TWP+LEF group; after treatment

the rats paw edema and arthritis index (AI) were evaluated; the levels of serum interleukin(IL)-1β

IL-6 and tumor necrosis factor alpha(TNF-α) were detected by radioimmunoassay method

serum interferon-gamma(IFN-γ)

IL-4 and prostaglandin E2(PGE2) by ELISA

and serum matrix metalloproteinases(MMP)-9 and MMP-2 by gelatin zymography assay. Result: ① Compared with AA model group

the hind paw swelling and AI in LEF group

TWP group and TWP+LEF group were significantly decreased (P<0.01)

especially in TWP+LEF group (P<0.05). ② The levels of serum IL-1β

TNF-α

IL-6

and PGE2 were notably higher in AA model group than control group (all P<0.01)

which was significantly decreased in three treatment groups (P<0.01 or P<0.05)

and their serum concentration was lower in TWP+LEF group than both LEF and TWP group (P<0.01 or P<0.05). ③ Compared with control group

the increase of serum IFN-γ and decrease of serum IL-4 was observed in model group (P<0.01). But the significant down-graduated expression of IFN-γ and up-graduated expression of IL-4 were found after treatment

particularly in TWP+LEF group (P<0.01). Conclusion: The combined use of TWP and LEF can significantly relieve the arthritis syndrome in AA rats by reducing the expression of serum cytokines

as well as inhibiting joint damage

and its therapeutic effect is better than used separately.

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