Effect of Yinao Jieyu Prescription on cAMP Signaling Pathway in Cortex and Hippocampus of Rats with Depression

TIAN Qing; TANG Qi-sheng; ZHAO Rui-zhen; LI Xiao-li

doi:CNKI:11-3495/R.20111202.1024.004

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Effect of Yinao Jieyu Prescription on cAMP Signaling Pathway in Cortex and Hippocampus of Rats with Depression

更新时间：2024-01-04

- Effect of Yinao Jieyu Prescription on cAMP Signaling Pathway in Cortex and Hippocampus of Rats with Depression
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 3, Pages: 121-124(2012)
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- DOI：CNKI:11-3495/R.20111202.1024.004
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- Published：2012
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TIAN Qing, TANG Qi-sheng, ZHAO Rui-zhen, et al. Effect of Yinao Jieyu Prescription on cAMP Signaling Pathway in Cortex and Hippocampus of Rats with Depression[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 121-124.
DOI：

TIAN Qing, TANG Qi-sheng, ZHAO Rui-zhen, et al. Effect of Yinao Jieyu Prescription on cAMP Signaling Pathway in Cortex and Hippocampus of Rats with Depression[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 121-124. DOI： CNKI:11-3495/R.20111202.1024.004.

摘要

目的: 观察抑郁大鼠模型脑内环磷酸腺苷(cAMP)通路的表达及中药颐脑解郁方的干预作用。方法: Wistar大鼠分为正常组、模型组、中药颐脑解郁方组、西药氟西汀组。模型组、中药颐脑解郁方组、西药氟西汀组在 21 d内采用慢性不可预见的温和性应激加孤养法(method of chronic unpredictable mild stress with isolated support)建立抑郁状态大鼠模型

模型组ig双蒸水

中药治疗组、西药对照组分别ig中药颐脑解郁方(6.2 g ·kg-1)、西药氟西汀(剂量2.33 mg ·kg-1)

采用RT-PCR实验方法

测定PDE4(3'

5'-cyclic-nucleotide phosphodiesterase)、CREB(cAMP response element binding protein)在抑郁模型大鼠脑内左前额叶皮质、海马的表达。结果: 模型组大鼠大脑额叶皮质及海马PDE4表达增强(P<0.05)

CREB表达明显低于正常组(P <0.05

P<0.01);中药颐脑解郁方及西药氟西汀干预后

额叶前侧皮质及海马PDE4的表达较模型组有明显的降低(P<0.05

P<0.01)。CREB表达明显增高(P <0.05

P<0.01)。结论: 中药颐脑解郁方干预后可明显调节PDE4及CREB在抑郁大鼠脑内的表达

提示中药颐脑解郁方的抗抑郁作用可能与调节cAMP信号转导通路有关。

Abstract

Objective: To study expression of brain cydic adenosine monophosphate(cAMP) pathway and intervention by Chinese medicine Yinao Jieyu prescription in depression rat model. Method: Depression model was established by method of Chronic unpredictable mild stress with isolated support

the expression of PDE4(3'

5'-cyclic-nucleotide phosphodiesterase) and cAMP response element binding protein(CREB) was assayed by (PCR)

in the rat brain model of depression left frontal cortex

hippocampus of the brain. Result: The hippocampus

prefrontal cortexand PDE4 were significantly higher than the normal group (P<0.05

P<0.01)

CREB expression was significantly lower than normal group (P<0.05

P<0.01). Chinese side Yinao Jieyu significantly after intervention in the brain regulated the expression of PDE4 and CREB (P<0.05

P<0.01). Conclusion: Yinao Jieyu prescription can regulate the expression of PDE4 and CREB in rat depression model

which may be related to regulating cAMP pathway.

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