Effect of Xuanbai Chengqi Decoction on CD14 and NF-B mRNA Expressions in Rat LPS-induced Acute Lung Injury Model

SU Zhong-hao; YANG Ai-dong; WANG Li-xia; GUO Yong-jie; WU Zhong-hua

doi:CNKI:11-3495/R.20111226.1610.002

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Effect of Xuanbai Chengqi Decoction on CD14 and NF-B mRNA Expressions in Rat LPS-induced Acute Lung Injury Model

更新时间：2024-01-04

- Effect of Xuanbai Chengqi Decoction on CD14 and NF-B mRNA Expressions in Rat LPS-induced Acute Lung Injury Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 5, Pages: 121-124(2012)
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- DOI：CNKI:11-3495/R.20111226.1610.002
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- Published：2012
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SU Zhong-hao, YANG Ai-dong, WANG Li-xia, et al. Effect of Xuanbai Chengqi Decoction on CD14 and NF-B mRNA Expressions in Rat LPS-induced Acute Lung Injury Model[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 121-124.
DOI：

SU Zhong-hao, YANG Ai-dong, WANG Li-xia, et al. Effect of Xuanbai Chengqi Decoction on CD14 and NF-B mRNA Expressions in Rat LPS-induced Acute Lung Injury Model[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 121-124. DOI： CNKI:11-3495/R.20111226.1610.002.

摘要

目的: 探讨宣白承气汤对脂多糖(lipopolysaccharide

LPS)诱导的急性肺损伤(acute lung injury

ALI)大鼠肺组织CD14和核转录因子-κB (nuclear factor-κB

NF-κB) mRNA表达的影响。方法: 健康雄性Wistar大鼠32只

随机分为正常对照组、模型对照组、地塞米松组、宣白承气汤组共4组

每组8只。模型组与各治疗组大鼠采用尾静脉注射LPS(6 mg·kg-1)复制ALI模型。正常对照组和模型组给等容积生理盐水ig

地塞米松组给药为5 mg·kg-1ip

宣白承气汤组给药为7 560 mg·kg-1ig。于3 d末次给药后采用实时荧光定量PCR法测定肺组织CD14和NF-κB mRNA表达。观察肺组织病理变化。结果: 与正常对照组比较

模型对照组的支气管肺泡灌洗液蛋白含量、肺体指数、CD14和NF-κB mRNA表达均明显升高(P<0.01)。与模型对照组比较

宣白承气汤组支气管肺泡灌洗液蛋白含量、肺体指数、CD14、NF-κB mRNA和CD14蛋白染色阳性细胞面积率表达均降低(P<0.05或P<0.01)。病理学观察

模型组大鼠肺泡腔内充满炎性渗出物和出血

宣白承气汤组大鼠肺组织病理改变明显轻于模型组

肺细支气管轻度间质性炎症。结论: 宣白承气汤能减轻内毒素致ALI大鼠肺组织损伤

对肺损伤有保护作用

其机制可能与其抑制肺组织CD14和NF-κB mRNA表达有关。

Abstract

Objective: The purpose of the study was to investigate the role of LPS (lipopolysaccharide) in acute lung injury (ALI) and protective effect of Xuanbai Chengqi(XBCQ) decoction. Method: Thirty two Wistar species male rats were randomly divided into 4 groups:normal control group

LPS treated group

dexamethasone group and XBCQ group

8 rats each group. LPS (6 mg·kg-1) was injected into the caudal vein of rats to perform ALI model. Normal controls and model group were given the same volume of physiological saline. The dosage of dexamethasone was 5 mg·kg-1ip. The dosage of XBCQ was 7 560 mg·kg-1ig. The experiment lasted for 3 d.CD14 and nuclear factor-κB(NF-κB) mRNA in lung tissue were measured by real time fluorescence quantitative PCR (RT-PCR). The pathological manifestation of the lung was observed. The mechanism was also analyzed. Result: Compared with normal group

the protein content of bronchoaleolar alveolar lavage fluid

body index of lung

expression of CD14 and NF-κB mRNA

expression of CD14 protein were greatly increased in LPS treated group. Compared with LPS treated group

the protein content of bronchoaleolar alveolar lavage fluid

body index of lung

expression of CD14 and NF-κB mRNA

expression of CD14 protein were decreased in XBCQ group(P<0.05 or P<0.01). Light microscope indicates large areas of pulmonary hemorrhage and necrosis were in model group. The pathological manifestations of XBCQ group were much more ameliorated than those of the model group. The inflammation of bronchiole was reduced. Conclusion: XBCQ decoction lessens the injury of lung tissue and has protective effects on rats with ALI induced by LPS. The mechanism is possibly related to the inhibition of the expressions of CD14 and NF-κB mRNA in injured lung tissues.

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