Objective:

2

To investigate the antihypertensive effect of Tianmu Jiangya powder and its related antihypertensive mechanism by using SHR rats as a model

and protein expressions provide an experimental basis for the clinical application of Tianmu Jiangya powder in the treatment of hypertension.

Method:

2

Sixty male SHR rats were randomly divided into six groups according to body weight after one week of adaptive feeding: model group

valsartan group (12 mg·kg

-1

)

captopril group (9 mg·kg

-1

)

hydrochlorothiazide group (6 mg·kg

-1

)

Tianmu Jiangya powder low and high-dose group (0.36

1.44 g·kg

-1

)

WKY rats were used as the normal group

and the intragastric administration lasted for 16 weeks. Softron BP-2010A intelligent non-invasive blood pressure meter was used to measure the systolic blood pressure (SBP)and heart rate (HR) of rat tail arteries. Adobe Photoshop CS5 software was used to analyze the left auricle and claw fixed selected areas to evaluate the effect on blood stasis syndrome. Vevo 2100 small animal ultrasound imaging system detects left ventricular ejection fraction (LVEF)

left ventricular shortening (FS)

left ventricular end-systolic volume (LVESV)

left ventricular end-diastolic volume (LVEDV)

left ventricular end-systole dimension (LVIDs)

left ventricular end-diastole dimension (LVIDd)

interventricular septum end-systolic depth (IVSs)

and interventricular septum end-diastolic depth (IVSd). Then the rats were sacrificed and the materials were taken (blood

heart

aorta

liver

kidney

tibia)

and the weight of heart

liver

kidney and tibia length were measured and recorded. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of the heart and thoracic aorta. Separation of serum and plasma

and determination of nitric oxide (NO) in serum by nitrate reductase method. Radioimmunoassay was used to detect plasma adrenaline/3 methoxyadrenaline (MN)

urea (UREA)

and uric acid (UA) contents. The expression of nitric oxide synthase(iNOS) and vascular endothelial growth factor(VEGF) protein in thoracic aorta of each group was detected and analyzed by immunohistochemical method.

Result:

2

Compared with normal group

the SBP and HR of the rats in model group were significantly increased (

P

<

0.05). The r value of the claw was significantly reduced and the g value was significantly increased at 8 and 16 weeks (

P

<

0.05). LVEF and FS significantly decreased

LVESV

LVIDs

IVSd increased significantly (

P

<

0.05). Heart weight

heart weight /tibia length

liver weight and liver weight /tibia length

plasma of MN

UREA

and UA contents significantly increased

and promoted the expression of iNOS and VEGF proteins in the aortic (

P

<

0.05). Compared with the model group

the Tianmu Jiangya powder administration group could continuously reduce SBP in SHR rats

maintain HR stability (

P

<

0.05)

significantly increase the claw of r value

lower the claw of g value(

P

<

0.05). LVEF

FS significantly increased

LVEDV

LVESV

LVIDd and LVIDs significantly decreased (

P

<

0.05)

significantly increased serum NO content

decreased liver weight

liver weight/tibia length

plasma MN

UREA

UA content (

P

<

0.05)

and down-regulated the expression of iNOS and VEGF protein in the aorta(

P

<

0.05).

Conclusion:

2

Tianmu Jiangya powder has a certain antihypertensive effect

and its mechanism may be mainly related to protecting heart function

improving vascular endothelial function

reducing catecholamines and sedative analgesia.