Objective:

2

To establish the fingerprints of standard decoction of Gentianae Macrophyllae Radix-dried products by different methods

and to evaluate the quality correlation.

Method:

2

HPLC

InertSustain C

18

chromatographic column(4.6 mm×250 mm

5 μm)

Gradient elution was performed for the mobile phase of acetonitrile-phospho

detection wavelength at 240 nm and flow rate of 0.8 mL·min

-1

and column temperature was 40 ℃. The quality correlation analysis of different methods for different kinds of Gentianae Macrophyllae Radix standard decoction was carried out from the aspects of chemical composition consistency

common chemical composition consistency

main chemical composition content and transfer rate.

Result:

2

The control fingerprint of Gentianae Macrophyllae Radix standard decoction was established. According to the peak matching data

there were 10 common peaks in the fingerprint of 15 batches of Gentianae Macrophyllae Radix standard decoction.Among the 10 common peaks

5 chemical constituents of loganic acid

6′-

O

-

β

-

D

-glucosyl gentiopicroside

swertiamarin

gentiopicroside and swertia glycosides were identified. The results of quality correlation analysis showed that the three different drying methods were consistent with the chemical composition and quantity of Gentianae Macrophyllae Radix standard decoction. But in terms of the content consistency of common chemical components and the transfer rate of main chemical components

the quality correlation between the products obtained from vacuum drying and the standard decoction was lower than that obtained from spray drying and freeze-drying.

Conclusion:

2

The fingerprint of different method of Gentianae Macrophyllae Radix standard decoction was established. Through the analysis of the mass correlation of chemical composition consistency

common chemical composition content consistency

main chemical composition content and transfer rate

the mass correlation between them was comprehensively reflected. It is suggested that spray drying or freeze drying should be used for the key drying process of Gentianae Macrophyllae Radix granules. This study provides a reference for the preparation process and quality control of Gentianae Macrophyllae Radix granules.