二陈汤加味对急性加重期COPD患者CXCL8及CXCR1/2蛋白表达的影响

尚立芝; 季书; 李耀洋; 毛梦迪; 王国强; 石龙涛; 张光远; 徐莉莉; 谢文英

doi:10.13422/j.cnki.syfjx.20192406

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二陈汤加味对急性加重期COPD患者CXCL8及CXCR1/2蛋白表达的影响

经典名方 | 更新时间：2021-02-09

- 二陈汤加味对急性加重期COPD患者CXCL8及CXCR1/2蛋白表达的影响
- Effect of Modified Erchentang on Expressions of Chemokine Receptor CXCR1/2 and CXCL8 in Patients with AECOPD
- 中国实验方剂学杂志 2019年25卷第24期页码：1-8
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
  
  尚立芝，硕士，教授，从事中医药作用机制研究，E-mail：lzshang2014@163.com
  王国强，博士，研究员，从事中医药免疫药理研究，E-mail：biowgq@126.com；
  谢文英，硕士，教授，从事中医药临床研究，E-mail：xiewenying1963@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81573881);河南省高等学校重点科研项目(15A360030);河南省科重点技攻关项目(182102311163);河南省教育科学“十三五”规划一般课题([2019]-JKGHYB-0101;[2019]-JKGHYB-0114);国家级大学生创新创业训练计划项目(S201810471013;S201910471010);河南中医药大学生创新学习项目(CXXM[2017]0252;CXXM[2019]0001)
- DOI：10.13422/j.cnki.syfjx.20192406
  中图分类号： R2-0; R22; R285.5; R289
- 纸质出版日期：2019-12-20，
  
  网络出版日期：2019-09-23，
  
  收稿日期：2019-06-28，
- 稿件说明：
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尚立芝, 季书, 李耀洋, 等. 二陈汤加味对急性加重期COPD患者CXCL8及CXCR1/2蛋白表达的影响[J]. 中国实验方剂学杂志, 2019,25(24):1-8.

Li-zhi SHANG, Shu JI, Yao-yang LI, et al. Effect of Modified Erchentang on Expressions of Chemokine Receptor CXCR1/2 and CXCL8 in Patients with AECOPD[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(24):1-8.
尚立芝, 季书, 李耀洋, 等. 二陈汤加味对急性加重期COPD患者CXCL8及CXCR1/2蛋白表达的影响[J]. 中国实验方剂学杂志, 2019,25(24):1-8. DOI： 10.13422/j.cnki.syfjx.20192406.

Li-zhi SHANG, Shu JI, Yao-yang LI, et al. Effect of Modified Erchentang on Expressions of Chemokine Receptor CXCR1/2 and CXCL8 in Patients with AECOPD[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(24):1-8. DOI： 10.13422/j.cnki.syfjx.20192406.

摘要

目的：

观察二陈汤加味对急性加重期慢性阻塞性肺疾病(AECOPD)患者CXC趋化因子配体8(CXCL8)及其受体CXCR1/2，巨噬细胞炎性蛋白2(MIP-2)蛋白表达的影响，评价二陈汤加味对AECOPD患者抗炎的作用与机制。

方法：

选取符合纳入标准的AECOPD患者200例，随机分成观察组和对照组各100例。2组均在西药治疗的基础上，对照组给予急支糖浆，观察组给予二陈汤加味，疗程14 d。酶联免疫吸附测定(ELISA)检测患者血浆中巨噬细胞炎性蛋白2(MIP-2)，CXCL8含量；蛋白免疫印迹法(Western blot)检测外周血单个核细胞(PBMCs)中CXCL8，CXCR1和CXCR2蛋白表达；免疫细胞化学染色检测PBMCs中CXCL8，CXCR1，CXCR2定位表达及阳性率。

结果：

治疗后观察组较同组治疗前血浆中CXCL8，MIP-2含量均显著减少(

0.05)，PBMCs中CXCL8，CXCR1，CXCR2蛋白表达均降低(

0.05，

0.01)。治疗后与对照组比较，观察组总有效率、肺FEV

均显著高于对照组(

0.05)，血浆MIP-2，CXCL8含量，CXCL8，CXCR1蛋白表达均显著低于对照组(

0.05)。

结论：

二陈汤加味对COPD有抗炎作用。其机制可能与下调CXCL8，CXCR1，CXCR2表达，减少CXCL8，MIP-2的合成与释放，抑制炎细胞的过多渗出与趋化，从而减轻炎症反应有关。

Abstract

Objective:

To observe the clinical efficacy of modified Erchentang on CXC chemokine ligand receptors (CXCR1/2)and their ligands CXCL8

macrophage inflammatory protein -2(MIP-2) in patients of chronic obstructive pulmonary disease(AECOPD)at acute exacerbation stage

and assess the effect and mechanism of modified Erchentang on anti-inflammatory in patients of AECOPD.

Method:

This study was a multicenter

randomized single blind

controlled trial. The authors selected 200 cases in conformity to the standards of AECOPD. The AECOPD patients were randomly divided into modified Erchentang group and control group. In addition to the western medicine

modified Erchentang was also given to the modified Erchentang group

and Jizhitangjiang was given to the control group for 14 days. Each group was observed for the alleviation of the symptoms. Euzyme-linked immunosorbent assay (ELISA) was used to determine the levels of CXCL8 and MIP-2 in the patients' plasma of all groups before and after treatment. Western blot were used to detect the levels of CXCR1

CXCR2 and CXCL8 protein in peripheral blood mononuclear cells(PBMCs). Immunocytochemistry (ICC) method was used to detect the expressions of CXCL8

CXCR1 and CXCR2 protein in PBMCs.

Result:

The level of CXCL8 in plasma

and the expressions of CXCR1

CXCR2 and CXCL8 mRNA and protein in the modified Erchentang group were decreased significantly than those in the control group(

0.05

0.01).

Conclusion:

Modified Erchentang has an anti-inflammatory effect on AECOPD. Its mechanism may be related to the down-regulation of the expressions of CXCL8

CXCR1 and CXCR2

the reduction of synthesis and release of CXCL8 and MIP-2

the inhibition of the chemotaxis and activity of inflammatory cells

and the prevention of inflammation progress.

关键词

慢性阻塞性肺疾病二陈汤外周血单个核细胞炎症趋化因子

Keywords

chronic obstructive pulmonary disease (COPD)Erchentangperipheral blood mononuclear cells (PBMCs)inflammationchemokine

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