商陆皂苷对脂多糖诱导的小鼠乳腺炎的影响

匡海学; 崔娜; 张雅男; 王知斌; 闫静

doi:10.13422/j.cnki.syfjx.20201302

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商陆皂苷对脂多糖诱导的小鼠乳腺炎的影响

药理 | 更新时间：2020-09-09

- 商陆皂苷对脂多糖诱导的小鼠乳腺炎的影响
- Effect of Esculentoside on Lipopolysaccharide-induced Mice Mastitis
- 中国实验方剂学杂志 2020年26卷第14期页码：65-71
- 作者机构：
  
  黑龙江中医药大学药学院，哈尔滨 150040
- 作者简介：
  
  匡海学，博士，教授，从事中药性味理论和药效物质基础研究，Tel：0451-82197188，E-mail：hxkuang@hljucm.net
  闫静，博士，教授，从事中药开发与分析研究，Tel：0451-87267050，E-mail：yanjing-002@163.com
- 基金信息：
  
  国家重点基础研究发展计划（973计划）项目(2013CB531801)
- DOI：10.13422/j.cnki.syfjx.20201302
  中图分类号： R2-0;R22;R285.5
- 纸质出版日期：2020-07-20，
  
  网络出版日期：2020-04-02，
- 稿件说明：
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匡海学,崔娜,张雅男等.商陆皂苷对脂多糖诱导的小鼠乳腺炎的影响[J].中国实验方剂学杂志,2020,26(14):65-71.

KUANG Hai-xue,CUI Na,ZHANG Ya-nan,et al.Effect of Esculentoside on Lipopolysaccharide-induced Mice Mastitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):65-71.
匡海学,崔娜,张雅男等.商陆皂苷对脂多糖诱导的小鼠乳腺炎的影响[J].中国实验方剂学杂志,2020,26(14):65-71. DOI： 10.13422/j.cnki.syfjx.20201302.

KUANG Hai-xue,CUI Na,ZHANG Ya-nan,et al.Effect of Esculentoside on Lipopolysaccharide-induced Mice Mastitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):65-71. DOI： 10.13422/j.cnki.syfjx.20201302.

摘要

目的

探究商陆皂苷对脂多糖诱导的小鼠乳腺炎的效果及作用机制。

方法

将BALB/c雌性小鼠随机分成正常组、模型组、地塞米松组（DEX，5.0 mg·kg

-1

）和商陆皂苷给药组（50，25，12.5 mg·kg

-1

），采用脂多糖（LPS）诱导产后雌小鼠(BALB/c)乳腺炎模型，分析小鼠乳腺组织的病理情况和中性粒细胞髓过氧化物酶（MPO）活性水平；测定乳腺组织中炎症因子的含量及氧化应激水平；通过蛋白免疫印迹法（Western blot）评估商陆皂苷对Toll样受体4（TLR4）/核转录因子-

B（NF-

B）信号通路蛋白的影响。

结果

与正常组比较，模型组的乳腺组织中有充血和瘀血等典型的乳腺炎变化，MPO水平升高，肿瘤坏死因子-

（TNF-

），白细胞介素-1

（IL-1

）和白细胞介素-6（IL-6）的表达水平显著升高（

0.01）；与LPS模型组比较，各商陆皂苷组能够显著改善小鼠乳腺组织的炎性损伤，降低嗜中性粒细胞的分泌，降低MPO的活性，促炎症因子TNF-

，IL-1

和IL-6的表达水平也明显下调，；升高超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px）酶活力，同时降低丙二醛（MDA）的水平（

0.05，

0.01），降低TLR4/NF-

B信号通路蛋白的表达（

0.05，

0.01）。

结论

商陆皂苷对脂多糖诱导的小鼠乳腺炎损伤有保护作用，其可能与抑制TLR4/NF-

B信号通路蛋白的表达有关。

Abstract

Objective

To explore the effect and mechanism of esculentoside on lipopolysaccharide-induced mastitis in mice.

Method

Female BALB/c mice were randomly divided into control group

model group

dexamethasone group (DEX

5.0 mg·kg

-1

) and esculentoside group (50

12.5 mg·kg

-1

). The mastitis model of postpartum female mice (BALB/c) induced by lipopolysaccharide (LPS) was used to analyze the pathological conditions of breast tissue and the activity of recombinant myeloperoxidase（MPO）

factor content and oxidative stress level. Western blot was used to evaluate the effect of esculentoside on Toll-like receptor4 (TLR4)/nuclear transcription factor (NF-

B) signaling pathway proteins.

Result

Compared with the normal group

the breast tissue of the model group had typical mastitis changes

such as hyperemia and congestion

the level of MPO increased

and the expression levels of tumor necrosis factor-

(TNF-

)

interleukin-1

(IL-1

) and interleukin-6 (IL-6) increased significantly (

0.01). Compared with LPS model group

esculentoside groups could significantly improve the inflammatory damage of mammary gland tissue

reduce the secretion of neutrophils and the activity of MPO

the expression levels of pro-inflammatory factors TNF-

IL-1

and IL-6 were also significantly down-regulated

the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were increased

while the level of malondialdehyde (MDA) was decreased

and the activation of TLR4/NF-

B signaling pathway was inhibited by esculentoside（

0.05，

0.01）.

Conclusion

Esculentoside have a protective effect on lipopolysaccharide-induced mastitis in mice

which may be related to the inhibition of TLR4/NF-

B signaling pathway protein expression.

关键词

商陆皂苷乳腺炎脂多糖（LPS）炎症因子Toll样受体4（TLR4）/核转录因子-κB（NF-κB）信号通路

Keywords

esculentosidemastitislipopolysaccharide (LPS)inflammatory factorsToll-like receptor (TLR4)/nuclear transcription factor-κB (NF-κB) pathway

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