Objective

2

To quickly analyze and identify the components in raw and rice-washed water products of

Atractylodes lancea

rhizoma （ALD） by ultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometry （UPLC-Q-TOF-MS）， and then find out the differential components before and after processing.

Method

2

TitanK C

18

column （2.1 mm×100 mm， 1.8 μm） was used with 0.1% formic acid aqueous solution （A）-acetonitrile （B） for gradient elution （0-0.01 min， 10%B； 0.01-12 min， 10%-25%B； 12-18 min， 25%-55%B； 18-30 min， 55%-70%B； 30-35 min， 70%-95%B； 35-37 min， 95%B； 37-37.1 min， 95%-10%B under negative ion mode and 0-0.01 min， 10%B； 0.01-10 min， 10%-56%B； 10-30 min， 56%-75%B； 30-35 min， 75%-95%B； 35-37 min， 95%B； 37-37.1 min， 95%-10%B under positive ion mode）. Electrospray ionization was selected for collection and detection in positive and negative ion modes with the scanning range of

m

/

z

100-1 250. Combining the reference substances， databases and related literature information， PeakView 1.2 software was used to identify the chemical components of ALD and its rice-washed water products. After normalized treatment by MarkerView™ 1.2.1， the MS data of each sample were analyzed with principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） in SICMA 14.1， and then the differential components before and after processing were screened according to the principle of variable importance in the projection （VIP） value

>

1 and

P

<

0.01 in

t

-test.

Result

2

A total of 56 components were identified from the raw and rice-washed water products of ALD， including 17 terpenoids， 8 polyacetylenes， 12 organic acids， 4 glycosides， 4 flavonoids and 11 others. There were 43 components in both， and 7 and 6 specific components respectively. The results of PCA and OPLS-DA showed that there were significant differences in the contents of the chemical components of ALD before and after processing， and 23 differential components such as atractylodin， atractylenolide Ⅰ， atractylenolide Ⅱ and wogonin were screened.

Conclusion

2

ALD mainly contains sesquiterpenoids， polyacetylenes and organic acids. The components of ALD are hydrolyzed before and after processing， and the content of active ingredients such as atractylodin increased. According to this， it can provide reference for the research on material basis of processing of ALD.