麸炒苍术多糖与正丁醇部位对脾虚大鼠代谢组学的影响

徐慧颖; 丁奕辉; 董昊; 赵雪岑; 柏阳; 才谦

doi:10.13422/j.cnki.syfjx.20230765

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麸炒苍术多糖与正丁醇部位对脾虚大鼠代谢组学的影响

药物代谢 | 更新时间：2023-10-20

- 麸炒苍术多糖与正丁醇部位对脾虚大鼠代谢组学的影响
  增强出版
- Effect of Polysaccharides and n-Butanol Fractions of Atractylodis Rhizoma Stir-fried with Bran on Metabonomics in Rats with Spleen Deficiency
- 中国实验方剂学杂志 2023年29卷第22期页码：112-120
- 作者机构：
  
  辽宁中医药大学药学院，辽宁大连 116600
- 作者简介：
  
  徐慧颖，硕士，助理实验师，从事中药药效物质基础研究，E-mail：13842057731@163.com
  才谦，博士，教授，从事中药化学研究，Tel：0411-85890125，E-mail：caiqianmail@sina.com
- 基金信息：
  
  国家自然科学基金项目(81973478);兴辽英才计划项目(XLYC2002004)
- DOI：10.13422/j.cnki.syfjx.20230765
  中图分类号： R22;R28;R969.1;O657
- 纸质出版日期：2023-11-20，
  
  网络出版日期：2023-04-20，
  
  收稿日期：2023-01-18，
- 稿件说明：
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徐慧颖,丁奕辉,董昊等.麸炒苍术多糖与正丁醇部位对脾虚大鼠代谢组学的影响[J].中国实验方剂学杂志,2023,29(22):112-120.

XU Huiying,DING Yihui,DONG Hao,et al.Effect of Polysaccharides and n-Butanol Fractions of Atractylodis Rhizoma Stir-fried with Bran on Metabonomics in Rats with Spleen Deficiency[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(22):112-120.
徐慧颖,丁奕辉,董昊等.麸炒苍术多糖与正丁醇部位对脾虚大鼠代谢组学的影响[J].中国实验方剂学杂志,2023,29(22):112-120. DOI： 10.13422/j.cnki.syfjx.20230765.

XU Huiying,DING Yihui,DONG Hao,et al.Effect of Polysaccharides and n-Butanol Fractions of Atractylodis Rhizoma Stir-fried with Bran on Metabonomics in Rats with Spleen Deficiency[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(22):112-120. DOI： 10.13422/j.cnki.syfjx.20230765.

摘要

目的

采用非靶向代谢组学技术探究麸炒苍术多糖与麸炒苍术正丁醇部位对脾虚大鼠血浆代谢产物的影响，进而阐明二者健脾作用机制。

方法

将40只雄性SD大鼠随机分为空白组、模型组、多糖组（0.075 6 g·mL

-1

）和正丁醇部位组（0.012 1 g·mL

-1

），每组10只。除空白组外，其余3组采用过度劳倦、饮食失节加苦寒泻下的复合因素造模方法复制脾虚大鼠模型，造模结束后多糖组和正丁醇部位组分别给予相应药液灌胃治疗7 d，空白组和模型组给予等体积生理盐水。采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法（UHPLC-Q-Orbitrap-MS）分析空白组、模型组、多糖组与正丁醇部位组大鼠血浆样品，并采用多元统计分析筛选差异代谢产物；采用京都基因与基因组百科全书（KEGG）数据库结合MetaboAnalyst 5.0软件对筛选出的差异代谢物进行代谢通路分析。

结果

多元统计分析结果显示，模型组与空白组、多糖组、正丁醇部位组间代谢产物均存在明显差异。空白组与模型组间共有380个差异代谢物，麸炒苍术多糖与麸炒苍术正丁醇部位分别回调了其中78个和57个差异代谢物。代谢通路富集结果显示，麸炒苍术正丁醇部位主要影响了甘氨酸、丝氨酸和苏氨酸代谢，丙氨酸、天冬氨酸和谷氨酸代谢及

-精氨酸、

-鸟氨酸代谢；麸炒苍术多糖主要影响了甘氨酸、丝氨酸和苏氨酸代谢，丙氨酸、天冬氨酸和谷氨酸代谢，生物素代谢，硫胺素代谢及三羧酸循环。

结论

麸炒苍术多糖与正丁醇部位均对脾虚大鼠的代谢异常有明显调节作用，其中正丁醇部位主要涉及氨基酸代谢，多糖除影响氨基酸代谢外，还参与调节能量代谢与辅因子和维生素代谢。

Abstract

Objective

To explore the regulatory effect of polysaccharides and n-butanol fractions of Atractylodis Rhizoma stir-fried with bran on the plasma metabolites of spleen-deficient rats， and then to elucidate their mechanisms of spleen-enhancing effects.

Method

Forty male SD rats were randomly divided into the blank group， model group， polysaccharide group （FD group， 0.075 6 g·mL

-1

·d

-1

）， n-butanol fractions group （FZ group， 0.012 1 g·mL

-1

·d

-1

）， with 10 rats in each group. Except the blank group， the other three groups used the compound factors of overwork， dietary disorders and intragastric administration of Sennae Folium decoction to replicate the rat model of spleen deficiency. After the end of modeling， the FD group and FZ group were given the corresponding medicinal solution by gavage for 7 d， meanwhile， the blank group and model group were given an equal volume of saline. The plasma samples from rats in the blank， model， FZ and FD groups were analyzed by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry （UHPLC-Q-Orbitrap-MS）， multivariate statistical methods were used to process the data and screen differential metabolites， and metabolic pathway enrichment analysis of the screened differential metabolites was performed using the Kyoto Encyclopedia of Genes and Genomes （KEGG)）database and MetaboAnalyst 5.0.

Result

The results of multivariate statistical analysis showed that there were significant differences in plasma metabolites between the model group and blank group， FZ group and model group， FD group and model group. There were 380 differential metabolites between the blank group and the model group， of which 78 and 57 were called back by polysaccharides and n-butanol fractions of Atractylodis Rhizoma stir-fried with bran， respectively. Metabolic pathway enrichment results showed that the n-butanol fractions mainly affected glycine， serine and threonine metabolism， alanine， aspartate and glutamate metabolism，

-arginine and

-ornithine metabolism， which were summarized as amino acid metabolism， while the polysaccharides mainly affected glycine， serine and threonine metabolism， alanine， aspartate and glutamate metabolism， tricarboxylic acid cycle， biotin metabolism and thiamine metabolism.

Conclusion

Both of polysaccharides and n-butanol fractions of Atractylodis Rhizoma stir-fried with bran have significant regulating effects on the metabolic abnormalities in spleen-deficient rats， in which the n-butanol fractions is mainly involved in amino acid metabolism， and the polysaccharides are involved in energy metabolism and cofactor and vitamin metabolism in addition to regulating amino acid metabolism.

关键词

苍术代谢组学多糖多元统计分析生物标志物氨基酸代谢正丁醇部位

Keywords

Atractylodis Rhizomametabonomicspolysaccharidesmultivariate statistical analysisbiomarkeramino acid metabolismn-butanol fractions

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