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1.中国中医科学院 中药研究所,北京 100700
2.河南中医药大学 药学院,郑州 450046
张美琴,硕士,从事中药炮制研究,E-mail:zhangmeiqin8@126.com
张村,博士,研究员,从事中药炮制、中药化学研究,Tel:010-64032658,E-mail:zhc95@163.com
收稿日期:2023-02-19,
网络出版日期:2023-04-21,
纸质出版日期:2023-07-05
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张美琴,王云,贾哲等.基于熵权-TOPSIS对不同产地桑白皮药材的品质评价[J].中国实验方剂学杂志,2023,29(13):130-139.
ZHANG Meiqin,WANG Yun,JIA Zhe,et al.Quality Evaluation of Mori Cortex from Different Producing Areas Based on Entropy Weight-TOPSIS Method[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(13):130-139.
张美琴,王云,贾哲等.基于熵权-TOPSIS对不同产地桑白皮药材的品质评价[J].中国实验方剂学杂志,2023,29(13):130-139. DOI: 10.13422/j.cnki.syfjx.20230865.
ZHANG Meiqin,WANG Yun,JIA Zhe,et al.Quality Evaluation of Mori Cortex from Different Producing Areas Based on Entropy Weight-TOPSIS Method[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(13):130-139. DOI: 10.13422/j.cnki.syfjx.20230865.
目的
2
应用熵权-逼近理想解排序法(TOPSIS)模型综合评价不同产地桑白皮药材的质量,为桑白皮的质量评价提供新方法。
方法
2
依据颜色、厚度、质地、粉性、粗皮残留5个关键指标,设计主观评分表,对桑白皮进行外观性状评价;建立高效液相色谱法(HPLC)指纹图谱及多成分(桑皮苷A、绿原酸、氧化白藜芦醇、桑皮苷C、桑根酮D、桑根酮C、桑辛素)的含量测定方法,采用化学计量学探寻桑白皮不同产地的差异性成分,采用TOPSIS综合评价不同产地桑白皮的质量;运用SPSS 22.0软件对桑白皮厚度、外观颜色与7个成分含量进行双变量相关性分析。
结果
2
颜色较淡,根皮越厚,质韧,粉性足,粗皮残留较少者得分较高,排名靠前者均为安徽产地的药材;建立的指纹图谱、含量测定方法稳定、可靠;偏最小二乘法-判别分析(PLS-DA)筛选出3个变量重要性投影(VIP)值
>
1的成分(桑皮苷A、桑根酮D、桑根酮C)对于桑白皮的产地差异有重要贡献;相关性分析表明,桑皮苷C与明度值(
L
*
)、总色度值(
E
*
ab
)呈明显正相关(
P
<
0.05),桑皮苷A与黄蓝色值(
b
*
)呈显著正相关(
P
<
0.01),桑根酮C与
b
*
呈显著负相关(
P
<
0.01),桑辛素与
L
*
呈明显负相关(
P
<
0.05);桑皮苷A、绿原酸、桑辛素与厚度呈显著负相关(
P
<
0.01),桑根酮D与厚度呈明显负相关(
P
<
0.05),桑根酮C与厚度呈显著正相关(
P
<
0.01);TOPSIS综合评分显示,来自安徽产地的桑白皮评分较好,排名靠前。
结论
2
不同产地的桑白皮质量存在较大差异性,产地距离较近的表现出外观、成分含量的特征相似性,颜色较浅、粗皮残留较少与药材品质呈正相关,其中安徽产地的桑白皮质量相对较优。
Objective
2
To evaluate the quality of Mori Cortex from different producing areas by the entropy weight-technique for order preference by similarity to an ideal solution(TOPSIS), and to provide a new evaluation method for the quality control of Mori Cortex.
Method
2
According to the five key indexes of color, thickness, texture, powdery and cortex remain, a subjective scoring table was designed to evaluate the appearance of Mori Cortex. High performance liquid chromatography(HPLC) was used to determine the fingerprint and the contents of multiple components(mulberroside A, chlorogenic acid, oxyresveratrol, mulberroside C, sanggenone D, sanggenone C, morusin), and chemometrics was used to explore the differential components of Mori Cortex from different habitats. On this basis, TOPSIS was used to comprehensively evaluate the quality of Mori Cortex from different habitats, and SPSS 22.0 software was used to carry out bivariate correlation analysis between thickness and appearance color with contents of seven components of Mori Cortex.
Result
2
Those with lighter color, thicker root bark, tougher texture, sufficient powder and less cortex remain scored higher, and the top five were all from Anhui. The established fingerprint and determination methods were stable and reliable. Partial least squares-discriminant analysis(PLS-DA) screened three components with the variable importance in the projection(VIP) value
>
1(mulberroside A, sanggenone D, sanggenone C), which made an important contribution to the difference in the origin of Mori Cortex. Correlation analysis showed that there was a significantly positive correlation between mulberroside C with lightness value(
L
*
) and total chromaticity value(
E
*
ab
) and mulberroside A with yellow-blue value(
b
*
)(
P
<
0.05,
P
<
0.01), a significantly negative correlation between sanggenone C with
b
*
and between morusin with
L
*
(
P
<
0.05,
P
<
0.01). And there was a significantly negative correlation between mulberroside A, chlorogenic acid, and morusin with thickness(
P
<
0.01), a clearly negative correlation between sanggenone D with thickness(
P
<
0.05), a significantly positive correlation between sanggenone C with thickness(
P
<
0.01). TOPSIS comprehensive scores showed that the samples from Anhui had a good score and ranked high.
Conclusion
2
There are great differences in the quality of Mori Cortex from different habitats, and those with the close habitats show similar characteristics in appearance and component content, and lighter color and less cortex were positively correlated with the quality. Among them, the quality of Mori Cortex from Anhui is relatively good.
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