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1.江西中医药大学 药学院,南昌 330004
2.范崔生全国名中医传承工作室,南昌 330006
[第一作者] 付小梅,教授,从事中药鉴定与质量评价研究,Tel:0791-87118776,E-mail:smilefxm@163.com
*吴志瑰,副教授,从事中药鉴定方面工作,Tel:0791-87118776,E-mail:nadinewzg@163.com
纸质出版日期:2020-05-05,
网络出版日期:2019-06-11,
收稿日期:2019-02-27,
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付小梅, 孙菡, 吴志瑰, 等. 药材南天竹的组织学鉴定[J]. 中国实验方剂学杂志, 2020,26(9):120-128.
Xiao-mei FU, Han SUN, Zhi-gui WU, et al. Histomorphological Identification of
付小梅, 孙菡, 吴志瑰, 等. 药材南天竹的组织学鉴定[J]. 中国实验方剂学杂志, 2020,26(9):120-128. DOI: 10.13422/j.cnkisyfjx.20191813.
Xiao-mei FU, Han SUN, Zhi-gui WU, et al. Histomorphological Identification of
目的:
2
通过对南天竹的根、茎、叶、果实进行系统的形态组织学研究,为其真伪鉴定提供依据。
方法:
2
采用性状鉴定方法对南天竹各药用部位进行外观特征描述;并采用石蜡切片技术和粉末制片技术对各药用部位如根、茎、叶、花、果实等横切面和粉末的显微特征进行观察,绘制墨线图并拍照。
结果:
2
发现该原植物形态特征与本草描述一致;药材性状为根、茎、叶、花、果实类生药特点;根横切面无髓部;茎横切面中可见皮层有断续成环的纤维束及木质部内侧的帽状纤维束;叶横切面中栅栏组织较为宽广,主脉维管束周围的纤维束成环;叶柄横切面中纤维束间断排列成环;果实横切面中可见多层石细胞组成的环带;根和茎的粉末特征中主要含有含晶石细胞;叶粉末中可见晶鞘纤维和不定式气孔;花粉末中可见花粉粒,具3孔沟,外壁网状雕纹明显,有较多的网纹细胞;果实粉末中可见大量分枝状石细胞及草酸钙方晶。
结论:
2
上述形态与显微鉴别特征有鉴定意义,为南天竹的真伪鉴别、质量标准及资源开发利用提供了科学依据。
Objective:
2
Histomorphological study of the Nandinae Radix
Nandinae Caulis
Nandinae Folium
Nandinae Fructus were conducted to provide the basis for the identification of its authenticity and falsehood.
Method:
2
The origin and macroscopic identification were used to describe the plant morphology and the appearance characteristics of all medicinal parts. The microscopic characteristics of the medicinal parts
such as roots
stems
leaves
flowers
fruits
were observed and photographed by paraffin section and powder preparation techniques.
Result:
2
It was found that the morphological characteristics of the original plant were consistent with the descriptions in herbaceous books. There was no pith on the cross-section of roots. In the transverse section of stems
there were intermittent circular fiber bundles in the cortex and a cap-shaped fibrous bundle in the inner part of the xylem. In the transverse section of the leaves
the palisade tissue was wider and the fiber bundles around the main vascular bundles formed a ring. In the transverse section of petiole
the fiber bundles were arranged intermittently into rings. In the transverse section of fruit
multi-layered sclereids formed a ring in the mesocarp. The powder characteristics of root and stem mainly contained crystalliferous sclereids. There were crystal sheath fibers and stomatal infinitive in the leaf powder
and pollen grains in the flower powder
with 3-hole grooves
obvious reticulate engraving pattern in the outer wall and more reticulate cells. There were a large number of branched sclereids and calcium oxalate square crystals in the fruit powder.
Conclusion:
2
The above-mentioned morphological and microscopic features have identification significance
and provide scientific basis for the authenticity identification
the quality standard and the utilization of resources of
Nandina domestica
.
南天竹组织来源鉴定性状鉴定显微鉴定根茎叶果实
Nandina domesticatissuesource identificationcharacter identificationmicroscopic identificationrootstemleavesfruit
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南京中医药大学.中药大辞典:下册[M].上海:上海科学技术出版社,2006:3.
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舒积成,彭财英,刘建群,等.南天竹化学成分及药理研究进展[J].中成药,2013,35(2):372-375.
彭财英,刘建群,张锐,等.南天竹种子中酚酸类成分[J].中国实验方剂学杂志,2014,20(23):95-98.
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吴启南.中药鉴定学实验指导[M].北京:中国医药科技出版社,2015:13.
国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:3.
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