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Identifizierung der Dan-Shen-Komponenten in vivo und in vitro basierend auf UPLC-Q-TOF-MS/MS

  • role： First author第一作者
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

  • Email：zhang.st@outlook.com
  • Introduction：张思彤，在读硕士，从事中药药效物质基础与体内过程研究，E-mail：zhang.st@outlook.com
ZHANG Sitong,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

HU Xianrun,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

LIU Wenkang,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

LEI Jinchun,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

CHENG Xuemei,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

WU Xiaojun,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

CHEN Wansheng,  
  • role： Corresponding author通信作者
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

  • Email：manlinli_cn@163.com
  • Introduction：李曼琳，博士，助理研究员，从事中药药效物质基础与药物代谢动力学研究，Tel：021-51322504，E-mail：manlinli_cn@163.com； *
LI Manlin*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

  • Email：wchcxm@163.com
  • Introduction：王长虹，博士，研究员，博士生导师，从事新药制剂与体内过程研究，Tel：021-51322511，E-mail：wchcxm@163.com
WANG Changhong*

Resümee

Ziel ist es, die chemische Zusammensetzung des Dan-Shen-Getränks und die Blutkomponenten nach oraler Verabreichung bei Ratten zu erläutern. Methode: Verwendung von Ultra-Hochleistungs-Flüssigkeitschromatographie gekoppelt mit Quadrupol-Flugzeit-Tandemmassenspektrometrie (UPLC-Q-TOF-MS/MS) in Kombination mit der PeakView 1.2-Software. Basierend auf Retentionszeit, Quasi-Molekularionenpeak, sekundären Fragmentionen und Literaturabgleich wurde eine systematische Charakterisierung und Identifikation der Dan-Shen-Wasserextrakte und der nach oraler Gabe im Blut auftretenden Komponenten bei Ratten durchgeführt. Weiterhin wurden die chemischen Bestandteile von gemeinsam mit Sandelholz und Amomum gekochten Dan-Shen-Wasserextrakten bestimmt, um die Herkunft der chemischen Bestandteile im Dan-Shen-Auszug klarzustellen. Ergebnisse: Insgesamt wurden 73 Verbindungen im Dan-Shen-Wasserextrakt identifiziert, darunter 21 Phenolsäuren, 23 Diterpene, 6 Flavonoide, 7 organische Säuren, 3 flüchtige Öle und 13 andere Typen. In der Arzneimittel-haltigen Rattenplasma wurden 36 Proto-Komponenten, die in das Blut gelangten, und 15 Metaboliten identifiziert. Die Hauptstoffwechselwege umfassen Reduktion, Hydratation, Hydroxylierung, Demethylierung, Methylierung und Sulfatierung. Diese Metaboliten stammen hauptsächlich von Danshenon- und Phenolsäurekomponenten. Fazit: Die Blutkomponenten des Dan-Shen-Wasserextrakts bestehen hauptsächlich aus Phenolsäuren und Danshenon-Komponenten, was möglicherweise die pharmakologische Wirkstoffbasis darstellt und als Referenz für Wirksamkeitsforschung, Qualitätskontrolle und klinische Anwendung des Dan-Shen-Getränks dienen kann.

Schlüsselwort

Dan-Shen-Getränk; chemische Bestandteile; Serumpharmakochemie; Metaboliten; Spaltungsmuster; Ultrahochleistungsflüssigkeitschromatografie-Quadrupol-Flugzeit-Tandem-Massenspektrometrie (UPLC-Q-TOF-MS/MS)

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