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1.河套学院 化学与环境工程系,内蒙古 巴彦淖尔 015000
2.宁夏葡萄酒与防沙治沙职业技术学院,银川 750199
3.宁夏大学 林业与草业学院,银川 750021
4.宁夏师范学院 资源环境与生命科学学院,宁夏 固原 756000
5.宁夏森林病虫防治检疫总站,银川 750001
Received:07 August 2023,
Published Online:24 October 2023,
Published:20 March 2024
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刘王锁,邱开阳,巴达日夫等.石质土生境银柴胡根系不同层次真菌多样性与土壤性质、药材主要成分的关系[J].中国实验方剂学杂志,2024,30(06):160-168.
LIU Wangsuo,QIU Kaiyang,BA Darifu,et al.Relationship Among Fungal Diversity at Different Levels of Root System of Stellaria dichotoma var. lanceolata, Soil Properties, and Main Components of Medicinal Materials in Lithosol Habitats[J].Chinese Journal of Experimental Traditional Medical Formulae,2024,30(06):160-168.
刘王锁,邱开阳,巴达日夫等.石质土生境银柴胡根系不同层次真菌多样性与土壤性质、药材主要成分的关系[J].中国实验方剂学杂志,2024,30(06):160-168. DOI: 10.13422/j.cnki.syfjx.20231415.
LIU Wangsuo,QIU Kaiyang,BA Darifu,et al.Relationship Among Fungal Diversity at Different Levels of Root System of Stellaria dichotoma var. lanceolata, Soil Properties, and Main Components of Medicinal Materials in Lithosol Habitats[J].Chinese Journal of Experimental Traditional Medical Formulae,2024,30(06):160-168. DOI: 10.13422/j.cnki.syfjx.20231415.
目的
2
探究石质土生境下银柴胡根系真菌与土壤因子及其药材主要成分之间的互作关系。
方法
2
采用高通量测序技术,测定银柴胡根系不同层次(根围、根际、根表和根内)真菌群落与根系(根际与根围)土壤性质,分析真菌群落与土壤性质、药材主要成分的关系。
结果
2
银柴胡根际土壤全磷、速效磷、碱解氮、可溶性有机碳及水分含量略高于根围,但差异不显著。银柴胡根系真菌优势门以子囊菌门(Ascomycota)为主,在根围-根际-根表-根内根系层次中,真菌多样性虽逐渐降低,但肉座菌目(Hypocreales)、可能新门unclassified_k_Fungi、柔膜菌目(Helotiales)及那提普斯壳目(Natipusillales)的成员丰度逐渐增加,其中肉座菌目是银柴胡根系最主要的真菌类群。结构方程模型(SEM)表明,根系土壤理化因子对真菌群落和药材主要成分起到了重要的调节作用,其中土壤全氮、碱解氮、水分和pH是主要的调节因子,土壤氮素含量是促进药材主要成分的关键,青霉属(
Penicillium
)真菌也是调节药材主要成分的关键真菌类群。
结论
2
银柴胡土壤理化性质对真菌群落和药材成分具有重要的作用,其根系肉座菌目真菌是较为重要的类群,而青霉菌属真菌对药材成分具有一定的介导作用。
Objective
2
To explore the interaction among root fungi of
Stellaria dichotoma
var.
lanceolata
, soil factors, and main components of medicinal materials in lithosol habitats.
Method
2
High-throughput sequencing technology was employed to determine the fungal community of the root system of
S. dichotoma
var.
lanceolata
at different levels (bulk soil, rhizosphere soil, rhizoplane soil, and root interior) and the soil properties of the root system (bulk and rhizosphere), and the relationship among the fungal community, soil properties, and the main components of medicinal materials was analyzed.
Result
2
The total phosphorus, available phosphorus, alkaline nitrogen, dissolved organic carbon, and soil water content in the rhizosphere soil of
S. dichotoma
var.
lanceolata
were slightly higher than those in the rhizosphere, but the difference was not significant. Ascomycota is the dominant phylum of root fungi in
S. dichotoma
var.
lanceolata
. In the progressive level of bulk-rhizosphere-rhizoplane-root interior system, although the fungal diversity gradually decreased, the abundance of Hypocreales, a new phylum (unclassified_ k_ Fungi), Helotiales, and Natipusilales gradually increased, among which Hypocreales is the most important fungal group in the root system of
S. dichotoma
var.
lanceolata
. The structural equation model (SEM) shows that the physicochemical factors of the root-soil play an important regulatory role in the fungal community and the main components of medicinal herbs, with soil total nitrogen, alkaline nitrogen, soil water content, and pH being the main regulatory factors. Soil nitrogen content is the key to promoting the main components of the medicinal herbs, and
Penicillium
fungi are the key fungal group to regulate the main components of the medicinal herbs.
Conclusion
2
It highlights that the physicochemical properties of the soil of
S. dichotoma
var.
lanceolata
play a crucial role in the fungal community and the components of medicinal materials. Hypocreales fungi in the root of
S. dichotoma
var.
lanceolata
were an important group, and
Penicillium
fungi had a certain role in mediating the components of medicinal materials.
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