“Ephedrine and pseudoephedrine were detected in the rhizomes of Ephedra sinica, but not in the rhizomes. The research progress in the field of active ingredients in traditional Chinese medicine was introduced. The research team established a validation system combining UHPLC-QqQ-MS and GC-MS chiral chromatography, and explored the molecular mechanism of SDR gene involvement in the asymmetric synthesis of alkaloids, laying the foundation for analyzing the stereoselective formation mechanism of ephedrine components and high-quality breeding.”