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Editor-in-Chief：WU Yiling
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National Administration of Traditional Chinese Medicine
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Institute of Traditional Chinese Medicine，China Academy of Chinese Medical Sciences；China Association of Chinese Medicine

CN：11-3495/R

ISSN：1005-9903

Publication Cycle：Semimonthly

Address：No.16，Nanxiao Street，Dongzhimen，Dongcheng District，Beijing

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E-mail：syfjx_2010@188.com

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Different Effects of Fresh and Dried Dendrobium Huoshanense on Chronic Atrophic Gastritis

ObjectiveTo compare the protective effects of water extracts from fresh and dried Dendrobium huoshanense on gastric mucosa in chronic atrophic gastritis （CAG）.MethodsMale SD rats （n=72） were randomly divided into 9 groups， with 8 rats in each group， which were normal group， model group， Yangwei Shu （4 g·kg-1） group， low-， medium-， and high-dose fresh D. huoshanense （3.5， 7， and 14 g·kg-1） groups， and low-， medium-， and high-dose dried D. huoshanense （0.7， 1.4， 2.8 g·kg-1） groups. The CAG rat model was successfully established by inducing with N-methyl-N'-nitro-N-nitrosoguanidine （MNNG） and other factors for a total of 11 weeks. Then， the rats were intervened with fresh and dried D. huoshanense for 4 weeks. The serum and gastric tissues of the rats were collected. The changes in gastric juice secretion volume and gastric acid pH value in each group were observed. The gastric mucosal injury was observed by naked eyes and hematoxylin-eosin（HE） staining. The gastric mucus secretion level was determined by Alcian blue and periodic acid-Schiff staining（AB-PAS） staining. The expression levels of tight junction proteins Occludin and ZO-1 in gastric tissues were determined by immunofluorescence. The expression levels of serum pepsinogen Ⅰ （PG Ⅰ）， pepsinogen Ⅱ （PG Ⅱ）， gastrin 17 （G-17）， interleukin-1β （IL-1β）， interleukin-6 （IL-6）， and tumor necrosis factor-α （TNF-α） were determined by enzyme-linked immunosorbent assay （ELISA）. The expression levels of aquaporin 1 （AQP1）， aquaporin 3 （AQP3）， and aquaporin 4 （AQP4） in gastric tissues were determined by Western blot.ResultsCompared with the normal group， the model group showed an obviously reduced gastric juice secretion volume （P0.05）， significantly increased gastric acid pH value （P0.01）， gastric mucosa with obvious atrophy， and a significantly reduced gastric mucus secretion volume （P0.01）. The expression of Occludin and ZO-1 in the gastric mucosal barrier was significantly decreased （P0.01）. The levels of PG Ⅰ and PG Ⅱ in the serum were obviously decreased （P0.05， P0.01）， and the levels of G-17， IL-1β， IL-6， and TNF-α were significantly increased （P0.01）. The expression level of AQP1 in the gastric tissue was significantly upregulated （P0.01）， and the expression levels of AQP3 and AQP4 were significantly downregulated （P0.01）. Compared with the model group， each drug administration group could improve the gastric mucosal atrophy of CAG model rats to varying degrees， obviously increase the gastric juice secretion volume of the model rats （P0.05， P0.01）， significantly decrease the gastric acid pH value （P0.01）， obviously increase the gastric mucus secretion volume （P0.05， P0.01）， obviously decrease the expression levels of G-17， IL-6， IL-1β， and TNF-α （P0.05， P0.01）， obviously increase the expression levels of Occludin， ZO-1， PG Ⅰ， and PG Ⅱ （P0.05， P0.01）， obviously upregulate the expression levels of AQP3 and AQP4 （P0.05， P0.01）， and obviously downregulate the expression level of AQP1 （P0.05， P0.01）.ConclusionThe water extracts of fresh and dried D. huoshanense can exert therapeutic effects on CAG by improving gastric mucosal injury， reducing inflammation， and regulating water metabolism. Moreover， the dried D. huoshanense has a better effect.

HU Mengqing, YANG Xinyu, GONG Weihan, XIE Huiqun, HAN Lan, PENG Daiyin

Different Effects of Fresh and Dried Dendrobium Huoshanense on Chronic Atrophic Gastritis
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Volume 31 期 23，2025 2025年第31卷第23期
    “最新研究发现，补阳还五汤通过调节JAK2/STAT3信号通路，改善糖尿病小鼠肾脏损伤和线粒体稳态。”

    LOU Feifei, ZHANG Xueqin, TIAN Xiaochao, WANG Yuehua, CHEN Zhiqiang

    Vol. 31, Issue 23, Pages: 1-11(2025) DOI: 10.13422/j.cnki.syfjx.20250536
    摘要：ObjectiveTo observe the regulatory effect of Buyang Huanwutang on mitochondrial homeostasis in renal tubular epithelial cells of spontaneous diabetic db/db mice through modulation of the Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） signaling pathway.MethodsThirty db/db mice were randomly divided into three groups （n=10 each）： model group， Buyang Huanwutang group （21.47 g·kg-1）， and Empagliflozin group （10 mg·kg-1）. Additionally， 10 db/m mice were used as the normal group. After 12 weeks of corresponding treatment， the kidney weight-to-body weight ratio （KW/BW）， 24-hour urinary protein quantification （24 h UTP）， urinary albumin-to-creatinine ratio （UACR）， serum creatinine （Scr）， N-acetyl-β-D-glucosaminidase （NAG）， neutrophil gelatinase-associated lipocalin （NGAL）， serum cystatin C （Cys C）， and kidney injury molecule-1 （KIM-1） were measured. Renal histopathological and morphological changes were observed by hematoxylin and eosin （HE） staining. The expression of KIM-1， apoptosis-related proteins， inflammatory cytokines， mitochondrial dynamics-related proteins， and JAK2/STAT3 pathway-related proteins was detected by Western blot and immunofluorescence （IF）. Superoxide dismutase （SOD） and malondialdehyde （MDA） levels were measured using enzyme-linked immunosorbent assay （ELISA）， and the expression of JAK2 and STAT3 mRNA in renal tissues was analyzed by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）.ResultsCompared with the normal group， KW/BW was significantly increased in the model group （P<0.05）， and 24 h UTP and UACR were also significantly increased （P<0.05， P<0.01）. Urinary KIM-1， NAG， NGAL， and Cys C levels were significantly increased （P<0.01）. HE staining revealed enlarged glomeruli， dilated renal tubules， and vacuolar degeneration in the model group. Serum interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） levels were significantly elevated （P<0.01）. The expression of B-cell lymphoma 2-associated X protein （Bax） and cleaved Caspase-3 was significantly upregulated （P<0.01）， while B-cell lymphoma 2 （Bcl-2） expression was significantly downregulated （P<0.01）. Serum MDA levels were markedly increased （P<0.05）， and SOD levels were significantly decreased （P<0.05）. The expression of mitochondrial dynamics-related proteins dynamin-related protein 1 （DRP1） and fission protein 1 （FIS1） was significantly upregulated （P<0.01）， while the fusion proteins optic atrophy 1 （OPA1） and mitofusin 2 （MFN2） were significantly downregulated （P<0.01）. Moreover， the expression of phosphorylated JAK2 （p-JAK2） and phosphorylated STAT3 （p-STAT3） was significantly increased （P<0.01）. Compared with the model group， there was no statistically significant difference in KW/BW between the Buyang Huanwutang and Empagliflozin groups， but 24 h UTP， UACR， urinary KIM-1， NAG， NGAL， and Cys C levels showed varying degrees of decrease （P<0.05， P<0.01）. IL-6 and TNF-α expression decreased， Bax and cleaved Caspase-3 expression was significantly reduced （P<0.01）， while Bcl-2 expression was significantly elevated （P<0.01）. MDA levels were markedly decreased （P<0.05， P<0.01）， SOD levels were significantly increased （P<0.01）， DRP1 and FIS1 expression was significantly downregulated （P<0.01）， and OPA1 and MFN2 expression was significantly upregulated （P<0.05， P<0.01）. The expression of p-JAK2 and p-STAT3 was significantly decreased （P<0.01）. Real-time PCR showed consistent trends， and renal pathological damage was alleviated.ConclusionBuyang Huanwutang can alleviate renal injury and apoptosis in db/db mice， improve renal inflammation and oxidative stress， and its mechanism may be related to the inhibition of excessive activation of the JAK2/STAT3 signaling pathway， thereby maintaining mitochondrial dynamics homeostasis.  
    关键词：Buyang Huanwutang;renal tubular epithelial cells;mitochondria;modulating the Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） pathway   
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    “据最新研究，黄连解毒汤能通过激活Nrf2/SLC7A11/GPX4信号通路改善脓毒症急性肺损伤。”

    LONG Min, LIU Keqin, YUE Yu, FEI Libo, SHAO Longgang, TONG Jiajia, QIN Huiling, ZHANG Xiaoyue, LI Tan, CHEN Huimin, ZHAO Qian, XIN Heng

    Vol. 31, Issue 23, Pages: 12-20(2025) DOI: 10.13422/j.cnki.syfjx.20241513
    摘要：ObjectiveTo study the improvement effect and mechanism of Huanglian Jiedutang on acute lung injury （ALI） in sepsis.MethodsExploring the pharmacological substance basis and potential mechanisms of Huanglian Jiedutang in improving ALI in sepsis through online pharmacology platforms； sixty SD rats were randomly divided into 6 groups： sham group， model group， positive group （10 mL·kg-1）， Huanglian Jiedutang low-dose group， Huanglian Jiedutang high-dose group（3.6、14.4 g·kg-1）， and Huanglian Jiedutang high-dose+nuclear factor-erythroid 2-related factor 2（Nrf2）inhibitor group （Huanglian Jiedutang+ML385）（14.4 g·kg-1+30 mg·kg-1）. A sepsis induced ALI model was created by cecal ligation and perforation surgery for 12 hours， followed by gavage administration. After 7 days of modeling， lung tissue was euthanized and isolated， and histopathological changes and expression of related key targets were observed.ResultsThe results showed that the high-frequency active ingredients of Huanglian Jiedutang in treating ALI were β- sitosterol， quercetin， stigmasterol， berberine， and berberine； By acting on 9 core targets including （GPX4）， Nrf2， Heme Oxygenase-1（HO-1）， TP53， mitogen-activated protein kinase 3（MAPK3）， tumor necrosis factor（TNF）， signal transducer and activator of transcription（STAT3）， interleukin（IL）-1β， MYC， and regulating signaling pathways such as ferroptosis， the effect of sepsis induced ALI is regulated. Huanglian Jiedutang can improve pathological damage such as alveolar interstitial edema and alveolar wall thickening in ALI rats with sepsis. The levels of malondialdehyde （MDA）， Fe2+， and reactive oxygen species （ROS） were significantly reduced（P<0.05）， while the levels of superoxide dismutase （SOD） and glutathione peroxidase （GSH） were significantly increased（P<0.05）. Electron microscopy and Prussian blue staining showed significant improvement in mitochondrial microstructure and iron deposition. Significantly increase the expression of Nrf2， solute carrier family 7 member 11（SLC7A11）， GPX4， and HO-1 proteins（P<0.05）. ML385 can significantly reverse the protective effect of Huanglian Jiedutang on sepsis induced ALI lung tissue.ConclusionHuanglian Jiedutang can improve ALI in sepsis through multiple pathways and targets， and its representative mechanism may be the activation of the Nrf2/SLC7A11/GPX4 signaling pathway.  
    关键词：Huanglian Jiedutang;sepsis;acute lung injury;nuclear factor-erythroid 2-related factor 2/solute carrier family 7 member 11/glutathione peroxidase 4（Nrf2/SLC7A11/GPX4）signal pathway;network pharmacology   
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    “最新研究发现，二冬汤通过激活IRS-1/PI3K/Akt信号通路，有效改善2型糖尿病小鼠糖脂代谢紊乱，减轻炎症反应和氧化应激。”

    LI Hao, JIANG Lijuan, ZHANG Wenfeng

    Vol. 31, Issue 23, Pages: 21-29(2025) DOI: 10.13422/j.cnki.syfjx.20251301
    摘要：ObjectiveTo investigate the therapeutic effects of Erdong Tang on hepatic glycolipid metabolism disorders in type 2 diabetes mellitus （T2DM） mice and explore the mechanism on the basis of the insulin receptor substrate 1 （IRS-1）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） pathway.MethodsA T2DM mouse model was established by high-fat and high-sugar feed combined with intraperitoneal injection of streptozotocin （STZ）. Mice successfully modeled were divided into a model group， a metformin group， and high-dose， medium-dose， and low-dose groups of Erdong Tang. The gavage doses for the metformin group and the high-dose， medium-dose， and low-dose groups of Erdong Tang were 0.15， 22.64， 11.32， 5.66 g·kg-1， respectively. At the same time， a blank group was set up， and the model and blank groups were administered with equal amounts of saline by gavage. After eight weeks of gavage administration， mice were measured for fasting blood glucose （FBG）， body mass， liver weight， liver coefficient， and an oral glucose tolerance test （OGTT） was conducted. Serum insulin （FINS）， interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α）， and reactive oxygen species （ROS） were measured by enzyme-linked immunosorbent assay （ELISA）， and insulin resistance index （HOMA-IR） was calculated. Total cholesterol （TC）， triglycerides （TG）， high-density lipoprotein （HDL）， and low-density lipoprotein （LDL） were detected. Hematoxylin-eosin （HE） staining was used to observe the histopathological damage of the liver. Western blot was used to detect the protein expression of IRS-1， PI3K， Akt， and p-Akt in liver tissue.ResultsCompared with those in the blank group， FBG， FINS， HOMA-IR， IL-6， TNF-α， ROS， TC， TG， LDL-C， liver weight， and liver coefficient were significantly increased in the model group （P<0.01）， and HDL-C， IRS-1， PI3K， Akt， p-Akt/Akt protein， and body mass were significantly reduced （P<0.01）. The pathological tissue in the liver showed unclear morphology of hepatic lobules， disordered arrangement of hepatocytes， enlarged nuclei of hepatocytes， unclear nuclear membrane， obvious fatty degeneration， and infiltration of inflammatory cells. Compared with that in the model group， the FBG， FINS， HOMA-IR， IL-6， TNF-α， ROS， TG， TC， and LDL-C content of mice was significantly reduced in the metformin group and the high-dose， medium-dose， and low-dose groups of Erdong Tang （P<0.05， P<0.01）. The expression of HDL-C， IRS-1， PI3K， and Akt protein was significantly increased in the metformin group and the high-dose and medium-dose groups of Erdong Tang （P<0.05， P<0.01）. The expression of p-Akt/Akt in mice was significantly increased in the metformin group and the high-dose， medium-dose， and low-dose groups of Erdong Tang （P<0.01）， and fatty degeneration and inflammatory cell infiltration were significantly improved in the pathological tissues of the mouse liver in each dosing group.ConclusionErdong Tang ameliorates glycolipid metabolism， inflammatory responses， and oxidative stress in T2DM mice， potentially by activating the IRS-1/PI3K/Akt signaling pathway.  
    关键词：Erdong Tang;type 2 diabetes mellitus （T2DM）;glycolipid metabolism;insulin receptor substrate 1 （IRS-1）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway   
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